Human Dkk-3 Antibody

Catalog #: MAB11181 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11181
MAB11181-SP

All Dkk-3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dkk-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dkk-3 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Dkk-1, recombinant mouse (rm) Dkk-2, or rmDkk-3 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 138728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Dkk-3
Ala22-Ile350
Accession # Q9UBP4.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human Dkk-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Dkk‑3 Biotinylated Antibody (Catalog # BAF1118)

Standard: Recombinant Human Dkk-3 Protein, CF (Catalog # 1118-DK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-3

Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists (1). Dkk-3 is a secreted monomer expressed in many normal human tissues, most strongly in heart, brain and spinal cord (1, 2), and during early embryonic development in the mouse (3). N-glycosylation at up to four sites preceding or between two conserved cysteine-rich motifs results in expression of a 45-65 kDa glycoprotein (1, 4). The cysteine-rich motifs contain 10 cysteines each, with prokineticin and colipase families containing sequences similar to those of the second motif (1, 5). Human Dkk-3 shows 82%, 88%, 85%, and 53% amino acid (aa) identity with mouse, bovine, canine, and chick Dkk-3, respectively, and 37-45% aa identity with other human Dkk family members. Several lines of evidence implicate Dkk-3 as a negative growth regulator. Dkk-3 is downregulated in many tumors as compared to normal cells, sometimes by loss of heterozygosity (4, 6). Downregulation by CpG hypermethylation in acute lymphoblastic leukemia is correlated with faster progression and shorter survival (7). Release of cultured cells from serum starvation results in downregulation of Dkk-3 in late G1 phase of the cell cycle (6). Over-expression of Dkk‑3 results in tumor cell-line-specific growth inhibition, induction of apoptosis, and decreased tumorigenicity in nude mice (2, 4, 6). The prototype Dickkopf member, Dkk-1, antagonizes Wnt family signaling by binding to Wnt receptors LRP5 and LRP6 (low-density lipoprotein receptor-related proteins) and promoting their internalization (1, 9, 10). Results are less straightforward for Dkk-3, where some studies show binding to LRP5/6 while others do not. These effects appear to be dependent on the cells and conditions used (1, 6-10).

References

Krupnik, V.E. et al. (1999) Gene 238:301.
Tsuji, T. et al. (2000) Biochem. Biophys. Res. Comm. 268:20.
Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
Hsieh, S.-Y. et al. (2004) Oncogene 23:9183.
Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
Tsuji, T. et al. (2001) Biochem. Biophys. Res. Comm. 289:257.
Roman-Gomez, J. et al. (2004) Br. J. Cancer 91:707.
Hoang, B.H. et al. (2004) Cancer Res. 64:2734.
Caricasole, A. et al. (2003) J. Biol. Chem. 278:37024.
Mao, B. et al. (2001) Nature 411:321.

Long Name

Dickkopf-3

Entrez Gene IDs

27122 (Human); 50781 (Mouse)

Alternate Names

dickkopf (Xenopus laevis) homolog 3; dickkopf 3; dickkopf homolog 3 (Xenopus laevis); Dickkopf-3; dickkopf-related protein 3; Dkk3; Dkk-3; hDkk-3; regulated in glioma; REIC; REICdkk-3; RIG; RIG-like 5-6; RIG-like 7-1