Human DLL1 Antibody

Catalog #: MAB18181
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB18181
MAB18181-SP

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DLL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DLL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DLL3 or rhDLL4 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 251123

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DLL1
Ser22-Gly540
Accession # AAG09716

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1.7-25 µg/mL

immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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DLL1 in HepG2 Human Cell Line. DLL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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DLL1 in Human Breast Cancer Tissue. DLL1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90‑100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6‑11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

References

Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
Karanu, F.N. et al. (2001) Blood 97:1960.
Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
Takahashi, Y. et al. (2003) Development 130:4259.
Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
Kiernan, A.E. et al. (2005) Development 132:4353.
Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
Limbourg, A. et al. (2007) Circ. Res. 100:363.
Purow, B.W. et al. (2005) Cancer Res. 65:2353.

Long Name

Delta-like 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Alternate Names

delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human DLL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
Br. J. Dermatol., 2011-05-17;164(6):1228-34.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
Delineation of a cellular hierarchy in lung cancer reveals an oncofetal antigen expressed on tumor-initiating cells.
Authors: Damelin M, Geles KG, Follettie MT, Yuan P, Baxter M, Golas J, Dijoseph JF, Karnoub M, Huang S, Diesl V, Behrens C, Choe SE, Rios C, Gruzas J, Sridharan L, Dougher M, Kunz A, Hamann PR, Evans D, Armellino D, Khandke K, Marquette K, Tchistiakova L, Boghaert ER, Abraham RT, Wistuba II, Zhou BB
Cancer Res., 2011-05-03;71(12):4236-46.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
Hypoxia-induced Jagged2 promotes breast cancer metastasis and self-renewal of cancer stem-like cells.
Authors: Xing F, Okuda H, Watabe M, Kobayashi A, Pai SK, Liu W, Pandey PR, Fukuda K, Hirota S, Sugai T, Wakabayshi G, Koeda K, Kashiwaba M, Suzuki K, Chiba T, Endo M, Mo YY, Watabe K
Oncogene, 2011-04-18;30(39):4075-86.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
Authors: Lasagni L, Ballerini L, Angelotti ML
Stem Cells, 2010-09-01;28(9):1674-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC