Human DLX5 Antibody

Catalog #: AF6710
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6710
AF6710-SP

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All DLX5 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DLX5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DLX5 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DLX5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DLX5
Asn216-Tyr289
Accession # P56178

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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DLX5 in Human Mesenchymal Stem Cells. DLX5 was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Sheep Anti-Human DLX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6710) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLX5

DLX5 (distal-less homeobox 5) is a 33-35 kDa member of the distal-less homeobox family of transcription factors. It is expressed in embryonic mesenchyme where it promotes cartilage and bone formation, and in nervous tissue, where it promotes interneuron development. Human DLX5 is 289 amino acids (aa) in length. It contains one DNA-binding homeobox domain (aa 137-196), and two phosphorylation sites at Ser34 and Ser217 that, when utilized, increases transcriptional activity. DLX5 likely forms homodimers, and is known to heterodimerize with MSX-2. Over aa 216-289, human DLX5 shares 99% aa identity with mouse DLX5.

Long Name

Distal-less Homeobox 5

Entrez Gene IDs

1749 (Human); 13395 (Mouse); 25431 (Rat)

Alternate Names

distal-less homeo box 5; distal-less homeobox 5; DLX5; homeobox protein DLX-5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human DLX5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Hydrogel mechanics are a key driver of bone formation by mesenchymal stromal cell spheroids
Authors: Jacklyn Whitehead, Katherine H. Griffin, Marissa Gionet-Gonzales, Charlotte E. Vorwald, Serena E. Cinque, J. Kent Leach
Biomaterials