Human DMPK Antibody

Name: Human DMPK Antibody
Brand: R&D Systems
SKU: MAB6967
Rating: 4 (1 reviews)

Catalog #: MAB6967 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6967
MAB6967-SP

Detection of Human DMPK by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DMPK Antibody Summary

Species Reactivity

Human

Specificity

Detects human DMPK in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 713931

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DMPK
Glu393-Thr543
Accession # Q09013

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DMPK by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DMPK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DMPK

DMPK (dystrophia myotonica protein kinase), also called DMK, MDPK or MT-PK (myotonin protein kinase) is an 80 kDa cytoplasmic protein of the ser/thr kinase superfamily (EC 2.7.11.1). The 639 amino acid (aa) human DMPK includes a protein kinase domain (aa 81-349), an AGC kinase C-terminal domain (aa 350-425) and a coiled-coil negative regulatory domain that mediates dimerization (aa 467-546). Within the region used as an immunogen, human DMPK shares 72% and 73% aa sequence identity with mouse and rat DMPK, respectively. This region is included within 12 isoforms that range in size from 530-634 aa. The most prominent, muscle-specific isoform phosphorylates phospholamban and is critical for cardiac contractility.

Long Name

Dystrophia Myotonica Protein Kinase

Entrez Gene IDs

1760 (Human); 13400 (Mouse); 308405 (Rat)

Alternate Names

DM1; DM1PK; DMK; DMKEC 2.7.11.1; DM-kinase; DMmyotonin protein kinase A; DMPK; dystrophia myotonica-protein kinase; MDPK; MDPKDM protein kinase; MT-PK; MT-PKmyotonic dystrophy associated protein kinase; Myotonic dystrophy protein kinase; myotonin-protein kinase; thymopoietin homolog