Human DNAM-1/CD226 Antibody

Catalog #: AF666
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF666
AF666-SP

Cell Adhesion Mediated by DNAM-1/CD226 and Neutral-ization by Human DNAM-1/ CD226 Antibody.

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All DNAM-1/CD226 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DNAM-1/CD226 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DNAM-1/CD226 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DNAM-1/CD226
Glu19-Asn247
Accession # Q15762

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN)

Neutralization

Measured by its ability to neutralize DNAM‑1-mediated adhesion of the COLO 205 human colorectal adenocarcinoma cell line. Shibuya, A. et al. (1996) Immunity 4:573. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 20 µg/mL Recombinant Human DNAM-1/CD226 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Adhesion Mediated by DNAM-1/CD226 and Neutral-ization by Human DNAM-1/ CD226 Antibody. Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DNAM-1/ CD226 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF666). The ND₅₀ is typically 0.5-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).

References

Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
Shibuya, A. et al. (1996) Immunity 4:573.
Bottino, C. et al. (2003) J. Exp. Med. 198:557.
Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
Morimoto, K. et al. (2007) Oncogene July 16 epub.
Shibuya, K. et al. (1999) Immunity 11:615.
Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
Shibuya, A. et al. (1998) J. Immunol. 166:1671.

Long Name

DNAX Accessory Molecule 1

Entrez Gene IDs

10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

Alternate Names

CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

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Citation for Human DNAM-1/CD226 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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