Human DNER Biotinylated Antibody

Catalog #: BAF3646
1 Citation Datasheet / COA / SDS

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BAF3646

Detection of Human DNER by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DNER Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DNER in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DNER
Arg29-His637
Accession # Q8NFT8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E13.5)

Immunocytochemistry

5-15 µg/mL

Immersion fixed A172 human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DNER by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3646) followed by streptavidin-HRP. A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNER

DNER (delta and notch-like EGF-related receptor; also BET) is a 120-150 kDa type I transmembrane glycoprotein that is reminescent of notch ligands such as Delta and Jagged. It is found on neuron dendrites and cell bodies but not on axons. Human DNER is 703 amino acids (aa) in length, and contains a 606 aa extracellular domain (ECD) (aa 35-640) plus a 76 aa cytoplasmic region. The ECD contains nine consecutive EGF-like domains, followed by one follistatin-like domain and a tenth EGF-like domain. DNER binding to notch-1 on glia induces morphological differentiation. Human DNER ECD is 89% aa identical to mouse DNER ECD.

Long Name

Delta/Notch-like EGF Repeat-containing Transmembrane

Entrez Gene IDs

92737 (Human); 227325 (Mouse)

Alternate Names

bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; DNER; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human DNER Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Biological and molecular heterogeneity of breast cancers correlates with their cancer stem cell content.
Authors: Pece S, Tosoni D, Confalonieri S, Mazzarol G, Vecchi M, Ronzoni S, Bernard L, Viale G, Pelicci PG, Di Fiore PP
Cell, 2010-01-08;140(1):62-73.
Species: Human
Sample Types: Whole Cells
Applications: ICC