Human DOK7 Antibody

Catalog #: MAB6398 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6398
MAB6398-SP

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Human DOK7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DOK7 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 651601

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DOK7
Ala179-Pro299
Accession # Q18PE1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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DOK7 in C2C12 Mouse Cell Line. DOK7 was detected in immersion fixed C2C12 mouse myoblast cell line differentiated into myotubes using Mouse Anti-Human DOK7 Monoclonal Antibody (Catalog # MAB6398) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DOK7

DOK7 (Downstream of kinase 7) is a 55 kDa member of the DOK family of cytoplasmic adaptor proteins. It links the acetylcholine receptor and the receptor tyrosine kinase MuSK in skeletal and cardiac muscle. Mutations can cause familial myasthenic syndromes. The 504 amino acid (aa) human DOK7 contains pleckstrin homology (aa 7-109) and phosphotyrosine-binding (PTK, aa 125-236) and SH2 domains, and a C-terminal nuclear export signal. Splicing isoforms of 255, 608 and 366 aa diverge at aa 175 or 500, or have 40 divergent aa replacing aa 1-178, respectively. Within aa 179-299, human DOK7 shares 92% and 93% aa identity with mouse and rat DOK7, respectively.

Long Name

Downstream of Tyrosine Kinase 7

Entrez Gene IDs

285489 (Human); 231134 (Mouse); 305448 (Rat)

Alternate Names

C4orf25; C4orf25chromosome 4 open reading frame 25; CMS1B; docking protein 7; DOK7; Dok-7; Downstream of tyrosine kinase 7; FLJ33718; FLJ39137; FLJ90556; protein Dok-7