Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody

Detection of Human, Mouse, and Rat Dopa Decarboxylase/DDC by Western Blot. Western blot shows lysates of mouse kidney tissue, PC-12 rat adrenal pheochromocytoma cell line, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Dopa Decarboxylase/DDC in IMR‑32 Human Cell Line. Dopa Decarboxylase/DDC was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Dopa Decarboxylase/DDC in Human Brain. Dopa Decarboxylase/DDC was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Dopa Decarboxylase/DDC in Mouse Brain. Dopa Decarboxylase/DDC was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Dopa Decarboxylase/DDC by Immunocytochemistry/Immunofluorescence DOPA decarboxylase is expressed non-uniformly in the CNS vasculature.(A–F) Coronal section of a P7 C57BL/6 brain stained for either GS lectin (green) to mark blood vessels or DOPA decarboxylase (DDC; magenta). Rightmost column shows merged images. Images are from the same brain section and correspond to cortex (A and B), striatum (C and D), and basal forebrain (E and F). DDC immunoreactivity is observed in blood vessels and in axon terminals, and in the latter it appears as distinct puncta in the cortex or more concentrated staining in the striatum. Yellow arrows in (C) indicate a large vein. Str, striatum; Ctx, cortex; AC, anterior commissure; Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30188322), licensed under a CC-BY license. Not internally tested by R&D Systems.