Human DPP9 Antibody

Catalog #: MAB54191 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB54191-100
MAB54191-SP

Detection of Human DPP9 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DPP9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DPP9 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 988515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DPP9
Arg2-Leu892
Accession # Q86TI2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DPP9 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DPP9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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DPP9 in HeLa Human Cell Line. DPP9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Human DPP9 by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for DPP9 at approximately 104 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPP9

DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).

References

Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
Qi, S.Y. et al. (2003) Biochem. J. 373:179.
Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.

Long Name

Dipeptidyl peptidase 9

Entrez Gene IDs

91039 (Human); 224897 (Mouse); 301130 (Rat)

Alternate Names

dipeptidyl peptidase 9; Dipeptidyl peptidase IV-related protein 2; dipeptidyl peptidase IV-related protein-2; Dipeptidyl peptidase IX; Dipeptidyl peptidase-like protein 9; dipeptidylpeptidase 9; dipeptidyl-peptidase 9; DKFZp762F117; DP9; DPLP9; DPP IX; DPP9; DPRP2; DPRP-2; EC 3.4.14.5; FLJ16073