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Human DPPA5/ESG1 Antibody

Catalog #: AF3125
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3125
AF3125-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DPPA5/ESG1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DPPA5/ESG1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse DPPA5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DPPA5/ESG1
Met1-Lys116
Accession # NP_001020461
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human DPPA5/ESG1
Immunocytochemistry
5-15 µg/mL
Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPA5/ESG1

DPPA5, also known as embryonic stem cell-specific gene 1 (ESG1), is a K homology RNA-binding (KH) domain containing protein that is expressed only in early embryos, germ cells and embryonic stem cells. It may be useful as a marker of pluripotent stem cells. Human DPPA5 shares approximately 66% and 74% amino acid sequence homology with mouse and rat DPPA5, respectively.

Long Name
Developmental Pluripotency Associated 5
Entrez Gene IDs
340168 (Human); 434423 (Mouse); 301101 (Rat)
Alternate Names
developmental pluripotency associated 5; developmental pluripotency-associated 5 protein; DPPA5; embryonal stem cell specific gene 1; Embryonal stem cell-specific gene 1 protein; ESG1; ESG1ESG-1; hDPPA5

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Citations for Human DPPA5/ESG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Single-Cell Transcriptomics Analysis of Human Small Antral Follicles
    Authors: X Fan, I Moustakas, M Bialecka, JS Del Valle, AW Overeem, LA Louwe, GSK Pilgram, LAJ van der We, H Mei, SM Chuva de S
    International Journal of Molecular Sciences, 2021-11-04;22(21):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution
    Authors: T Messmer, F von Meyenn, A Savino, F Santos, H Mohammed, ATL Lun, JC Marioni, W Reik
    Cell Rep, 2019-01-22;26(4):815-824.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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