Human DPPII/QPP/DPP7 Antibody

Catalog #: MAB3438 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3438
MAB3438-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DPPII/QPP/DPP7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DPPII/QPP/DPP7 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 398011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DPPII/QPP/DPP7
Gly22-Leu492
Accession # ABM85563

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Recombinant Human DPPII/QPP/DPP7 (0.1 µg/mL, Catalog # 3438-SE) cleavage of the fluorogenic peptide substrate KP-AMC (10 µM). The Neutralization Dose (ND₅₀) is typically 0.12 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPII/QPP/DPP7

Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The human DPP7 precursor consists of a signal peptide (aa 1‑21) and a mature chain (aa 22‑492). The purified rhDPP7 is active against Lys‑Pro‑AMC and Lys‑Ala‑AMC. Its activity against Lys‑Pro‑AMC is approximately 10‑fold of that against Lys‑Ala‑AMC under otherwise identical conditions.

References

Underwood, R. et al. (1999) J. Biol. Chem. 274:34053.
Maes, M.B. et al. (2005) Biochem. J. 386:315.
Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
Lankas, G.R. et al. (2005) Diabetes 54:2988.

Long Name

Dipeptidyl-peptidase II

Entrez Gene IDs

29952 (Human); 83768 (Mouse); 83799 (Rat)

Alternate Names

carboxytripeptidase; Dipeptidyl aminopeptidase II; dipeptidyl arylamidase II; dipeptidyl peptidase 2; Dipeptidyl peptidase 7; Dipeptidyl peptidase II; dipeptidylpeptidase 7; dipeptidyl-peptidase 7; dipeptidyl-peptidase II; DPP II; DPP2; DPP7; DPPII; EC 3.4.14.2; QPP; Quiescent cell proline dipeptidase