Human E2F-4 Antibody

Catalog #: AF5139 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5139
AF5139-SP

Detection of Human E2F‑4 by Western Blot.

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Human E2F-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human E2F-4 in Western bots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human E2F-4
Lys178-Asp306
Accession # Q16254

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human E2F‑4 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human E2F-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5139) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for E2F-4 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E2F-4

E2F-4 (viral E2-associated factor 4) is a 50 kDa member of the E2F/DP family of transcription factors. It is ubiquitously and constitutively expressed, and forms a DNA-binding E2F heterodimeric complex with DP-1. This complex, when associated with p130/RBL2 and a series of LIN-numbered proteins, represses cell cycle‑dependent genes during G0 and G1. Human E2F-4 is 413 amino acids (aa) in length and contains a poly-Pro region (aa 9‑12), a DNA-binding domain (aa 16‑85), a dimerization segment (aa 86‑181), poly-Ser region (aa 307‑327), and an RBL1/RBL2 binding domain (aa 390‑407). There is one potential alternate start site at Met261. Over aa 178‑306, human E2F-4 shares 80% and 83% aa identity with mouse and canine E2F-4, respectively.

Long Name

E2F Transcription Factor 4

Entrez Gene IDs

1874 (Human); 104394 (Mouse)

Alternate Names

E2F transcription factor 4, p107/p130-binding; E2F4; E2F-4; E2F-4p107/p130-binding protein; transcription factor E2F4