Human EBI2 Antibody

Catalog #: MAB102721 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB102721-100
MAB102721-SP

Detection of EBI2/GPR183 in HEK293 Human Cell Line Transfected with Human EBI2/GPR183 and eGFP by Flow Cytometry.

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Human EBI2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EBI2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 486617

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived transfected with recombinant human EBI2
Accession # P32249

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EBI2/GPR183 in HEK293 Human Cell Line Transfected with Human EBI2/GPR183 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human EBI2/GPR183 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EBI2/GPR183 Monoclonal Antibody (Catalog # MAB102721) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on isotype control antibody (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI2

Epstein-Barr virus-induced G-protein coupled receptor 2, also known as EBI2 or GPR183 is a G-protein coupled receptor (GPCR) expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, and monocytes/macrophages. The GPR183 gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of the Burkitt's lymphoma cell line BL41. GPR183 collaborates with CXCR5 to mediate B cell homing within a lymph node, probably by forming a heterodimer with CXCR5 that affects its interaction with CXCL13

Long Name

Epstein-Barr Virus Induced Gene 2

Entrez Gene IDs

1880 (Human); 321019 (Mouse); 679975 (Rat)

Alternate Names

EBI2; EBI2Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupledreceptor); EBV-induced G-protein coupled receptor 2; Epstein-Barr virus-induced G-protein coupled receptor 2; G protein-coupled receptor 183; GPR183; G-protein coupled receptor 183