Human EBI2 Antibody

Catalog #: MAB10272 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10272-100
MAB10272-SP

Detection of EBI2 in HEK293 Human Cell Line Transfected with Human EBI2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EBI2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EBI2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 486633

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived transfected withrecombinant human EBI2
Accession # P32249

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EBI2 in HEK293 Human Cell Line Transfected with Human EBI2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) EBI2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2A Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of EBI2 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human EBI2 Monoclonal Antibody (Catalog # MAB10272, filled histogram) or Mouse IgG2A Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBI2

Epstein-Barr virus-induced G-protein coupled receptor 2, also known as EBI2 or GPR183 is a G-protein coupled receptor (GPCR) expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, and monocytes/macrophages. The GPR183 gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of the Burkitt's lymphoma cell line BL41. GPR183 collaborates with CXCR5 to mediate B cell homing within a lymph node, probably by forming a heterodimer with CXCR5 that affects its interaction with CXCL13

Long Name

Epstein-Barr Virus Induced Gene 2

Entrez Gene IDs

1880 (Human); 321019 (Mouse); 679975 (Rat)

Alternate Names

EBI2; EBI2Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupledreceptor); EBV-induced G-protein coupled receptor 2; Epstein-Barr virus-induced G-protein coupled receptor 2; G protein-coupled receptor 183; GPR183; G-protein coupled receptor 183