Human eIF4G1 Antibody

Catalog #: AF4018 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4018
AF4018-SP

Detection of Human eIF4G by Western Blot.

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Human eIF4G1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human eIF4G1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human eIF4G1
Leu1401-Asn1599
Accession # Q04637

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human eIF4G by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human eIF4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for eIF4G at approximately 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF4G1

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is an important member of the translation initiation mechanism. It serves as a scaffold for other initiation factors needed for the recruitment of mRNA to the ribosome, including eIF4E, eIF4A, eIF3, and PABP (poly (A)-binding protein). The eIF4E/eIF4G interaction binds to the 5’ m7GTP mRNA cap, while the PABP/eIF4G interaction binds to the poly (A) tail of mRNA. eIF4A is an ATP-dependent RNA helicase. These interactions link the process of poly (A) binding, mRNA cap recognition, and unwinding of secondary structure. In addition when eIF4G is cleaved by a viral protease the majority of host cell mediated translation is diverted to an IRES (internal ribosomal entry site) mediated initiation of translation.

Long Name

Eukaryotic Translation Initiation Factor 4 gamma 1

Entrez Gene IDs

1981 (Human)

Alternate Names

DKFZp686A1451; EIF4F; eIF-4G 1; eIF4G1; EIF-4G1; eIF-4-gamma 1; EIF4-gamma; EIF4GI; EIF4Gp220; eucaryotic translation initiation factor 4G; eukaryotic translation initiation factor 4 gamma 1; eukaryotic translation initiation factor 4 gamma, 1; P220