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Human EMP3 Antibody

Catalog #: MAB4505
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4505
MAB4505-SP
Detection of EMP3 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EMP3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EMP. Stains human EMP3 cell transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 439949
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human EMP3
Met1-Glu163
Accession # P54852
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin and PBMC monocytes fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of EMP3 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of EMP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Intracellular Staining by Flow Cytometry View Larger

Detection of EMP3 in PBMC monocytes by Flow Cytometry. PBMC monocytes were stained with Mouse Anti-Human EMP3 Monoclonal Antibody (Catalog # MAB4505, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMP3

Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20‑25 kDa molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3.

Long Name
Epithelial Membrane Protein 3
Entrez Gene IDs
2014 (Human); 13732 (Mouse); 81505 (Rat)
Alternate Names
EMP3; EMP-3; epithelial membrane protein 3; HNMP-1; Protein YMP; YMP; YMPHematopoietic neural membrane protein 1

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Citation for Human EMP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype
    Authors: N Thornton, V Karamatic, L Tilley, CA Green, CL Tay, RE Griffiths, BK Singleton, F Spring, P Walser, AG Alattar, B Jones, R Laundy, JR Storry, M Möller, L Wall, R Charlewood, CM Westhoff, C Lomas-Fran, V Yahalom, U Feick, A Seltsam, B Mayer, ML Olsson, DJ Anstee
    Nat Commun, 2020-07-16;11(1):3569.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization

FAQs

  1. Why do you recommend intracellular staining with a flow antibody to a membrane expressed protein?

    • The antibody may recognize an intracellular portion of the protein,or an abundance of the protein may be in an intracellular location. In our hands, the intracellular staining worked better than surface staining.

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