Human ENPP-7/Alk-SMase Antibody

Catalog #: MAB4924 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4924
MAB4924-SP

Detection of Human ENPP‑7/Alk‑SMase by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ENPP-7/Alk-SMase Antibody Summary

Species Reactivity

Human

Specificity

Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-5, recombinant mouse ENPP-7 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 560514

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
Pro23-Ser439
Accession # Q6UWV6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ENPP‑7/Alk‑SMase by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human ENPP-7/Alk-SMase Monoclonal Antibody (Catalog # MAB4924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ENPP-7/Alk-SMase at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-7/Alk-SMase

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is a 60 kDa GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%‑36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is down‑regulated in some human colorectal carcinomas. The 416 amino acid (aa) mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Alternate Names

Alkaline sphingomyelin phosphodiesterase; alkaline sphingomyelinase; Alk-SMase; EC 3.1.4.12; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; ENPP7; ENPP-7; Intestinal alkaline sphingomyelinase; MGC50179; NPP7; NPP-7