Human ERMAP Antibody

Catalog #: MAB4928 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4928
MAB4928-SP

Detection of Human ERMAP by Western Blot.

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Human ERMAP Antibody Summary

Species Reactivity

Human

Specificity

Detects human ERMAP in direct ELISAS.

Source

Monoclonal Mouse IgG_2B Clone # 767504

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ERMAP
His30-Ala155
Accession # Q96PL5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ERMAP by Western Blot. Western blot shows lysates of human erythrocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ERMAP Monoclonal Antibody (Catalog # MAB4928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ERMAP at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERMAP

ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60‑66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30‑155) that contains one V-type Ig-like domain (aa 30‑140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103‑475. Over aa 30‑155, human ERMAP is 56% aa identical to mouse ERMAP. In contrast to human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.

Long Name

Erythroblast Membrane-associated Protein

Entrez Gene IDs

114625 (Human); 27028 (Mouse); 298485 (Rat)

Alternate Names

ERMAP; erythroblast membrane-associated protein (RD and SC blood groups); erythroblast membrane-associated protein (Scianna blood group); erythroblast membrane-associated protein; erythroid membrane-associated protein; hERMAP; MGC118810; MGC118811; PRO2801; Radin blood group (Rd); Radin blood group antigen; Radin blood group; RD; RDMGC118812; SC; Scianna blood group (Sc); Scianna blood group antigen; Scianna blood group; SCMGC118813