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Human FABP2/I-FABP Quantikine ELISA Kit

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27 Citations 3 Reviews Datasheet / COA / SDS
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Human FABP2/I-FABP ELISA Linearity
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Citations (27)
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Summary Sample Values Precision Recovery Linearity Product Datasheets Preparation and Storage Background Related Research Areas

Human FABP2/I-FABP Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
6.21 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human FABP2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human FABP2 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=36)1015389-2129455
EDTA plasma952377-2049444
Heparin plasma (n=36)797230-1800381


Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Urine (n=10)18490ND-632
ND=Non-detectable

Cell Culture Supernates - Human peripheral blood leukocytes (PBL) were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were then cultured unstimulated or stimulated with 10 μg/mL PHA for 1 or 6 days. Aliquots of the cell culture supernates were removed and assayed for levels of human FABP2. No detectable levels were observed.

Cell Lysates - LoVo human colorectal adenocarcinoma cells were cultured in Kaighn's supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 1.5 g/L sodium bicarbonate and grown until confluent. An aliquot of the cell lysate was removed, assayed for human FABP2, and measured 82.3 pg/mg.


Product Summary

The Quantikine Human FABP2/I-FABP Immunoassay is a 4.5 hour solid phase ELISA designed to measure FABP2 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FABP2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FABP2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human FABP2 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human FABP2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 77.5 207 425 80.5 216 446
Standard Deviation 3.14 7.24 12.4 8.94 17.4 26.6
CV% 4.1 3.5 2.9 11.1 8.1 6

Recovery

The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 96-104
Cell Lysis Buffer (n=2) 83 81-85
EDTA Plasma (n=4) 97 85-102
Heparin Plasma (n=4) 95 96-104
Serum (n=4) 101 97-106
Urine (n=4) 90 85-95

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FABP2/I-FABP ELISA Linearity

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name:
Fatty Acid-Binding Protein 2/Intestinal FABP
Entrez Gene IDs:
2169 (Human); 25598 (Rat)
Alternate Names:
FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human FABP2/I-FABP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Biomarkers In Prediction of Acute Mesenteric Ischaemia: a prospective multicentre study (BIPAMI study): a study protocol
    Authors: Tamme, K;Acosta, S;Biloslavo, A;Björck, M;Casian, D;Damaskos, D;Forbes, A;Kase, K;Kisand, K;Lakbar, I;Mihnovitš, V;Murruste, M;Mändul, M;Nuzzo, A;Padar, M;Starkopf, J;Visconti, D;Reintam Blaser, A;
    BMC surgery
    Species: Human
    Sample Types: Plasma
  2. Association of Blast Exposure in Military Breaching with Intestinal Permeability Blood Biomarkers Associated with Leaky Gut
    Authors: Liu, Q;Wang, Z;Sun, S;Nemes, J;Brenner, LA;Hoisington, A;Skotak, M;LaValle, CR;Ge, Y;Carr, W;Haghighi, F;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  3. HIV-associated gut microbial alterations are dependent on host and geographic context
    Authors: Rocafort, M;Gootenberg, DB;Luévano, JM;Paer, JM;Hayward, MR;Bramante, JT;Ghebremichael, MS;Xu, J;Rogers, ZH;Munoz, AR;Okello, S;Kim, JH;Sentongo, R;Wagubi, R;Lankowski, A;Maruapula, S;Zhao, G;Handley, SA;Mosepele, M;Siedner, MJ;Kwon, DS;
    Nature communications
    Species: Human
    Sample Types: Plasma
  4. Adults on pre-exposure prophylaxis (tenofovir-emtricitabine) have faster clearance of anti-HIV monoclonal antibody VRC01
    Authors: Huang, Y;Zhang, L;Karuna, S;Andrew, P;Juraska, M;Weiner, JA;Angier, H;Morgan, E;Azzam, Y;Swann, E;Edupuganti, S;Mgodi, NM;Ackerman, ME;Donnell, D;Gama, L;Anderson, PL;Koup, RA;Hural, J;Cohen, MS;Corey, L;McElrath, MJ;Gilbert, PB;Lemos, MP;
    Nature communications
    Species: Human
    Sample Types: Serum
  5. Organoids transplantation attenuates intestinal ischemia/reperfusion injury in mice through L-Malic acid-mediated M2 macrophage polarization
    Authors: Zhang, FL;Hu, Z;Wang, YF;Zhang, WJ;Zhou, BW;Sun, QS;Lin, ZB;Liu, KX;
    Nature communications
    Species: Human
    Sample Types: Plasma
  6. Enteric fungi protect against intestinal ischemia-reperfusion injury via inhibiting the SAA1-GSDMD pathway
    Authors: Chen, Y;Han, B;Guan, X;Du, G;Sheng, B;Tang, X;Zhang, Q;Xie, H;Jiang, X;Tan, Q;Chen, S;Wang, J;Chen, W;Xiao, W;
    Journal of advanced research
    Species: Mouse
    Sample Types: Feces
  7. Exosomal circEZH2_005, an intestinal injury biomarker, alleviates intestinal ischemia/reperfusion injury by mediating Gprc5a signaling
    Authors: Zhang, W;Zhou, B;Yang, X;Zhao, J;Hu, J;Ding, Y;Zhan, S;Yang, Y;Chen, J;Zhang, F;Zhao, B;Deng, F;Lin, Z;Sun, Q;Zhang, F;Yao, Z;Liu, W;Li, C;Liu, KX;
    Nature communications
    Species: Human
    Sample Types: Plasma
  8. Citrulline, Intestinal Fatty Acid-Binding Protein and the Acute Gastrointestinal Injury Score as Predictors of Gastrointestinal Failure in Patients with Sepsis and Septic Shock
    Authors: Tyszko, M;Lemańska-Perek, A;Śmiechowicz, J;Tomaszewska, P;Biecek, P;Gozdzik, W;Adamik, B;
    Nutrients
    Species: Human
    Sample Types: Plasma
  9. Microbiota-derived tryptophan metabolites indole-3-lactic acid is associated with intestinal ischemia/reperfusion injury via positive regulation of YAP and Nrf2
    Authors: FL Zhang, XW Chen, YF Wang, Z Hu, WJ Zhang, BW Zhou, PF Ci, KX Liu
    Journal of Translational Medicine, 2023-04-18;21(1):264.
    Species: Human
    Sample Types: Serum
  10. The Clinical Significance of Serum Biomarkers of the Intestinal Barrier in Systemic Sclerosis: A Cross-Sectional Study
    Authors: A Stec, M Maciejewsk, M Zaremba, K Paralusz-S, M Michalska, L Rudnicka, M Sikora
    Journal of personalized medicine, 2023-04-18;13(4):.
    Species: Human
    Sample Types: Serum
  11. Effects of Flavonoid-Rich Orange Juice Intervention on Major Depressive Disorder in Young Adults: A Randomized Controlled Trial
    Authors: J Choi, JH Kim, M Park, HJ Lee
    Nutrients, 2022-12-28;15(1):.
    Species: Human
    Sample Types: Serum
  12. Reduced circulating FABP2 in patients with moderate to severe COVID-19 may indicate enterocyte functional change rather than cell death
    Authors: G Assante, A Tourna, R Carpani, F Ferrari, D Prati, F Peyvandi, F Blasi, A Bandera, A Le Guennec, S Chokshi, VC Patel, IJ Cox, L Valenti, NA Youngson
    Scientific Reports, 2022-11-05;12(1):18792.
    Species: Human
    Sample Types: Plasma
  13. Altered gut microbiota and its metabolites correlate with plasma cytokines in schizophrenia inpatients with aggression
    Authors: H Deng, L He, C Wang, T Zhang, H Guo, H Zhang, Y Song, B Chen
    BMC Psychiatry, 2022-09-27;22(1):629.
    Species: Human
    Sample Types: Serum
  14. Gut biomolecules (I-FABP, TFF3 and lipocalin-2) are associated with linear growth and biomarkers of environmental enteric dysfunction (EED) in Bangladeshi children
    Authors: MM Hasan, MA Gazi, S Das, SM Fahim, F Hossaini, AR Khan, J Ferdous, MA Alam, M Mahfuz, T Ahmed
    Scientific Reports, 2022-08-16;12(1):13905.
    Species: Human
    Sample Types: Plasma
  15. Plasma Microbiome in COVID-19 Subjects: An Indicator of Gut Barrier Defects and Dysbiosis
    Authors: R Prasad, MJ Patton, JL Floyd, S Fortmann, M DuPont, A Harbour, J Wright, R Lamendella, BR Stevens, GY Oudit, MB Grant
    International Journal of Molecular Sciences, 2022-08-15;23(16):.
    Species: Human
    Sample Types: Plasma
  16. Intestinal fatty acid binding protein (I-FABP) and CXC3L1 evaluation as biomarkers for patients at high-risk for coeliac disease in Johannesburg, South Africa
    Authors: A Gandini, T De Maayer, C Munien, K Bertrand, R Cairns, A Mayne, MP Gededzha, ES Mayne
    Cytokine, 2022-07-13;157(0):155945.
    Species: Human
    Sample Types: Serum
  17. Intestinal fatty acid binding protein is a disease biomarker in paediatric coeliac disease and Crohn's disease
    Authors: M Logan, M MacKinder, CM Clark, A Kountouri, M Jere, UZ Ijaz, R Hansen, P McGrogan, RK Russell, K Gerasimidi
    BMC gastroenterology, 2022-05-23;22(1):260.
    Species: Human
    Sample Types: Plasma
  18. Translocating lipopolysaccharide correlates with the severity of enterovirus A71-induced HFMD by promoting pro-inflammation and viral IRES activity
    Authors: Y Wang, K Dan, X Xue, X Yang, X Feng, Q Yang, J Yang, B Chen
    Gut pathogens, 2021-11-22;13(1):69.
    Species: Human
    Sample Types: Serum
  19. Impact of 12-week exercise program on biomarkers of gut barrier integrity in patients with coronary artery disease
    Authors: V Feng, KK Bawa, S Marzolini, A Kiss, P Oh, N Herrmann, KL Lanctôt, D Gallagher
    PLoS ONE, 2021-11-19;16(11):e0260165.
    Species: Human
    Sample Types: Serum
  20. Allergy-related diseases and early gut fungal and bacterial microbiota abundances in children
    Authors: K Schei, MR Simpson, T Øien, S Salamati, K Rudi, RA Ødegård
    Clinical and translational allergy, 2021-06-28;11(5):e12041.
    Species: Human
    Sample Types: Serum
  21. Identifying a biological signature of prenatal maternal stress
    Authors: JM Keane, AS Khashan, FP McCarthy, LC Kenny, JM Collins, SM O'Donovan, JR Brown, JF Cryan, TG Dinan, G Clarke, SM O'Mahony
    JCI Insight, 2021-01-25;0(0):.
    Species: Human
    Sample Types: Serum
  22. Long-lasting severe immune dysfunction in Ebola virus disease survivors
    Authors: A Wiedemann, E Foucat, H Hocini, C Lefebvre, BP Hejblum, M Durand, M Krüger, AK Keita, A Ayouba, S Mély, JC Fernandez, A Touré, S Fourati, C Lévy-March, H Raoul, E Delaporte, L Koivogui, R Thiébaut, C Lacabaratz, Y Lévy
    Nat Commun, 2020-07-24;11(1):3730.
    Species: Human
    Sample Types: Serum
  23. Epinephrine treatment but not time to ROSC is associated with intestinal injury in patients with cardiac arrest
    Authors: KA Krychtiuk, B Richter, M Lenz, P Hohensinne, K Huber, C Hengstenbe, J Wojta, G Heinz, WS Speidl
    Resuscitation, 2020-06-06;0(0):.
    Species: Human
    Sample Types: Serum
  24. Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
    Authors: LB Giron, CE Tanes, MH Schleimann, PA Engen, LM Mattei, A Anzurez, M Damra, H Zhang, K Bittinger, F Bushman, A Kossenkov, PW Denton, H Tateno, A Keshavarzi, AL Landay, M Abdel-Mohs
    Mucosal Immunol, 2020-03-09;0(0):.
    Species: Human
    Sample Types:
  25. Gut permeability, inflammation, and bone density across the menopause transition
    Authors: A Shieh, M Epeldegui, AS Karlamangl, GA Greendale
    JCI Insight, 2020-01-30;0(0):.
    Species: Human
    Sample Types: Plasma
  26. Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
    Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
    Sci Rep, 2019-02-19;9(1):2304.
    Species: Human
    Sample Types: Plasma
  27. Relation between postoperative ileus and anastomotic leakage after colorectal resection: a post hoc analysis of a prospective randomized controlled trial
    Authors: E G Peters
    Colorectal Dis, 2017-07-01;0(0):.
    Species: Human
    Sample Types: Plasma

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Reviews for Human FABP2/I-FABP Quantikine ELISA Kit

Average Rating: 4.7 (Based on 3 Reviews)

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Human FABP2/I-FABP Quantikine ELISA Kit
By Adriana Trajtman on 10/20/2017
Sample Tested: blood and respiratory
Human FABP2/I-FABP Quantikine ELISA Kit
By Anonymous on 09/07/2017
Sample Tested: Serum

The Quantikine ELISA kit worked as expected with low % CV's, and reproducible results.

Human FABP2/I-FABP Quantikine ELISA Kit DFBP20
Human FABP2/I-FABP Quantikine ELISA Kit
By Anonymous on 02/08/2017
Sample Tested: Human Plasma
Human FABP2/I-FABP Quantikine ELISA Kit DFBP20