Human FABP4/A-FABP Antibody

Catalog #: AF3150
19 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3150
AF3150-SP

Detection of Human, Mouse, and Rat FABP4/A‑FABP by Western Blot.

4 Images

All FABP4/A-FABP Antibodies

Product Details

Citations (19)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FABP4/A-FABP Antibody Summary

Species Reactivity

Human

Specificity

Detects human FABP4/A‑FABP in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse (rm) FABP4 is observed, approximately 30% cross‑reactivity with recombinant human (rh) FABP3 is observed, and less than 5% cross‑reactivity with rhFABP1, -2, -5, -6, -7, -8, -9, and rmFABP9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FABP4/A‑FABP
Cys2-Ala132
Accession # P15090

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (Catalog # MAB3150).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FABP4/A-FABP DuoSet ELISA Kit (Catalog # DY3150-05) for convenient development of a sandwich ELISA or the Human FABP4 Quantikine ELISA Kit (Catalog # DFBP40) for a complete optimized ELISA.

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat FABP4/A‑FABP by Western Blot. Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

FABP4/A‑FABP in Human Bladder Cancer Tissue. FABP4/A-FABP was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

View Larger

Detection of Human and Mouse FABP4/A‑FABP by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A-FABP at approximately 19 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

ELISA

View Larger

Human/Mouse FABP4/A‑FABP ELISA Standard Curve. Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.

Long Name

Fatty Acid-Binding Protein 4

Entrez Gene IDs

2167 (Human); 11770 (Mouse); 79451 (Rat)

Alternate Names

Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human FABP4/A-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
Filter your results:

Filter by:

Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs
Authors: Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al.
iScience
SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer
Authors: Shijia Bi, Zunpeng Liu, Zeming Wu, Zehua Wang, Xiaoqian Liu, Si Wang et al.
Protein & Cell
Fetal baboon sex-specific outcomes in adipocyte differentiation at 0.9 gestation in response to moderate maternal nutrient reduction.
Authors: Tchoukalova Y, Krishnapuram R, White U, Burk D, Fang X, Nijland M, Nathanielsz P
Int J Obes (Lond), 2013-06-10;38(2):224-30.
Palmitate decreases migration and proliferation and increases oxidative stress and inflammation in smooth muscle cells: role of the Nrf2 signaling pathway
Authors: Josefa Girona, Roser Rosales, Paula Saavedra, Lluís Masana, Joan-Carles Vallvé
American Journal of Physiology-Cell Physiology
A vicious loop of fatty acid-binding protein 4 and DNA methyltransferase 1 promotes acute myeloid leukemia and acts as a therapeutic target
Authors: F Yan, N Shen, J X Pang, N Zhao, Y W Zhang, A M Bode et al.
Leukemia
FABP4-mediated lipid accumulation and lipolysis in tumor-associated macrophages promote breast cancer metastasis
Authors: Yorek, M;Jiang, X;Liu, S;Hao, J;Yu, J;Avellino, A;Liu, Z;Curry, M;Keen, H;Shao, J;Kanagasabapathy, A;Kong, M;Xiong, Y;Sauter, ER;Sugg, SL;Li, B;
eLife
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology
Authors: Gomez-Sequeda, N;Mendivil-Perez, M;Jimenez-Del-Rio, M;Lopera, F;Velez-Pardo, C;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: ICC
CD36+ Fibroblasts Secrete Protein Ligands That Growth-Suppress Triple-Negative Breast Cancer Cells While Elevating Adipogenic Markers for a Model of Cancer-Associated Fibroblast
Authors: K Jabbari, Q Cheng, G Winkelmaie, S Furuta, B Parvin
International Journal of Molecular Sciences, 2022-10-22;23(21):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites
Authors: C Bosc, N Broin, M Fanjul, E Saland, T Farge, C Courdy, A Batut, R Masoud, C Larrue, S Skuli, N Espagnolle, JC Pagès, A Carrier, F Bost, J Bertrand-M, J Tamburini, C Récher, S Bertoli, V Mansat-De, S Manenti, JE Sarry, C Joffre
Nat Commun, 2020-08-13;11(1):4056.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration
Authors: C Liang, Z Liu, M Song, W Li, Z Wu, Z Wang, Q Wang, S Wang, K Yan, L Sun, T Hishida, Y Cai, JCI Belmonte, P Guillen, P Chan, Q Zhou, W Zhang, J Qu, GH Liu
Cell Res., 2020-07-31;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Patient Adipose Stem Cell-Derived Adipocytes Reveal Genetic Variation that Predicts Antidiabetic Drug Response
Authors: W Hu, C Jiang, D Guan, P Dierickx, R Zhang, A Moscati, GN Nadkarni, DJ Steger, RJF Loos, C Hu, W Jia, RE Soccio, MA Lazar
Cell Stem Cell, 2019-01-10;24(2):299-308.e6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Firemasterï¿½ 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (Ppar?) on the adipocyte protein 2 (aP2) promoter
Authors: EWY Tung, S Ahmed, V Peshdary, E Atlas
PLoS ONE, 2017-04-24;12(4):e0175855.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Identification of multipotent stem cells in human brain tissue following stroke
Authors: K Tatebayash, Y Tanaka, A Nakano-Doi, R Sakuma, S Kamachi, M Shirakawa, K Uchida, H Kageyama, T Takagi, S Yoshimura, T Matsuyama, T Nakagomi
Stem Cells Dev, 2017-04-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand
Sci Rep, 2016-07-22;6(0):30311.
Species: Human
Sample Types: Whole Cells
Applications: IHC

Gene expression profile analysis of human mesenchymal stem cells from herniated and degenerated intervertebral discs reveals different expression of osteopontin.
Authors: Marfia G, Navone S, Di Vito C, Tabano S, Giammattei L, Di Cristofori A, Gualtierotti R, Tremolada C, Zavanone M, Caroli M, Torchia F, Miozzo M, Rampini P, Riboni L, Campanella R
Stem Cells Dev, 2014-10-29;24(3):320-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis.
Authors: Xia H, Bodempudi V, Benyumov A, Hergert P, Tank D, Herrera J, Braziunas J, Larsson O, Parker M, Rossi D, Smith K, Peterson M, Limper A, Jessurun J, Connett J, Ingbar D, Phan S, Bitterman P, Henke C
Am J Pathol, 2014-03-13;184(5):1369-83.
Species: Human
Sample Types: Whole Cells
Applications: IHC

Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
Authors: Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J
PLoS ONE, 2013-03-22;8(3):e59252.
Species: Human
Sample Types: Whole Cells
Applications: ICC

A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Authors: Klim JR, Li L, Wrighton PJ
Nat. Methods, 2010-11-14;7(12):989-94.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Bisphenol S- and bisphenol A-induced adipogenesis of murine preadipocytes occurs through direct peroxisome proliferator-activated receptor gamma activation
Authors: S Ahmed, E Atlas
International Journal of Obesity