Human FABP6 Antibody

Catalog #: AF3880
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3880
AF3880-SP

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FABP6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FABP6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP7, and recombinant mouse FABP9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FABP6
Ala2-Ala128
Accession # P51161

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FABP6

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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FABP6 in T84 Human Cell Line. FABP6 was detected in immersion fixed T84 human colon carcinoma cell line using Goat Anti-Human FABP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP6

Fatty acid binding protein-6 (FABP6), also known as ileal bile acid binding protein (I-BABP) and gastrotropin, is a 15 kDa cytoplasmic protein that belongs to the FABP family. It is expressed in ileal epithelium and multiple other tissues. Alternate transcription promoters generate two transcript variants, encoding a 128 aa and a 177 aa residue protein. Human FABP6 isoform 2 contains 128 amino acid residues and is believed to be acetylated on Ala2. It binds both fatty acids and bile acids and has roles in fatty acid transport and metabolism. The amino acid sequence of human FABP6 is 80%, 78%, and 75% aa identical to that of mouse, canine, and porcine FABP6, respectively.

Long Name

Fatty Acid-Binding Protein 6

Entrez Gene IDs

2172 (Human); 16204 (Mouse); 25440 (Rat)

Alternate Names

FABP6; fatty acid binding protein 6, ileal; Fatty acid-binding protein 6; Gastrotropin; I-15P; I-15PIntestinal 15 kDa protein; IBABP; I-BABPIntestinal bile acid-binding protein; I-BALB; I-BAP; ILBP; ILBP3; ILBPGT; ileal bile acid binding protein; ILLBP; ILLBPIleal lipid-binding protein; illeal lipid-binding protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human FABP6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling
Authors: W Gu, H Wang, X Huang, J Kraiczy, PNP Singh, C Ng, S Dagdeviren, S Houghton, O Pellon-Car, Y Lan, Y Nie, J Zhang, KK Banerjee, EJ Onufer, BW Warner, J Spence, E Scherl, S Rafii, RT Lee, MP Verzi, D Redmond, R Longman, K Helin, RA Shivdasani, Q Zhou
Cell Stem Cell, 2021-09-27;0(0):.
Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels
Authors: Claudia Loebel, Robert L. Mauck, Jason A. Burdick
Nature Materials