Human FAM3C Antibody

Catalog #: AF3084 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3084
AF3084-SP

Detection of Human FAM3C by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FAM3C Antibody Summary

Species Reactivity

Human

Specificity

Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse FAM3C is observed and 5% cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, and rhFAM3D is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FAM3C
Gln25-Asp227
Accession # Q92520

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FAM3C by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FAM3C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FAM3C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM3C

FAM3C, also known as predicted Osteoblast protein, is a secreted 22 kDa glycoprotein belonging to the cytokine-like protein family. Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. FAM3 is ubiquitously expressed in multiple tissues. The amino acid sequence of mature human FAM3C is 94% and 98% identical to that of mouse and canine FAM3C, respectively.

Long Name

Family With Sequence Similarity 3, Member C

Entrez Gene IDs

10447 (Human); 27999 (Mouse); 312159 (Rat)

Alternate Names

D6WSU176e; FAM3C; family with sequence similarity 3, member C; GS3876; ILEI; ILEIInterleukin-like EMT inducer; predicted osteoblast protein