Human FANCD2 Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC9369G-100UG
Detection of FANCD2 in HeLa Human Cell Line by Flow Cytometry.
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Human FANCD2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FANCD2 in direct ELISAs and Western blots.
Source
Monoclonal Rabbit IgG Clone # 1290D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human FANCD2
Glu11-Glu230
Accession # Q9BXW9
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of FANCD2 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of FANCD2 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human FANCD2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC9369G, filled histogram) or isotype control antibody (Catalog # IC1051G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FANCD2

Fanconi anemia group D2 protein, encoded by the FANCD2 gene, is a member of the Fanconi anemia complementation (FANC) group that also includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCE, FANCF, FANCG, and FANCL. The members of the Fanconi anemia complementation group share little sequence similarities.

Long Name
Fanconi Anemia, Complementation Group D2
Entrez Gene IDs
2177 (Human); 211651 (Mouse); 312641 (Rat)
Alternate Names
DKFZp762A223; FA4; FACD; FAD; FAD2; FA-D2; FADFAD2; FANCD; FANCD2; Fanconi anemia complementation group D2; Fanconi anemia group D2 protein; Fanconi anemia, complementation group D2; FLJ23826; FPN1; HFE4; IREG1; Protein FACD2; SLC11A3

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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