Human Ferritin Antibody

Name: Human Ferritin Antibody
Brand: R&D Systems
SKU: MAB93541
Rating: 5 (2 reviews)

Catalog #: MAB93541 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB93541-100
MAB93541-SP

Detection of Human Ferritin by Western Blot.

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Human Ferritin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ferritin in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 962609

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Ferritin synthetic peptide
Accession # P02794

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Immunocytochemistry

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Ferritin by Western Blot. Western blot shows lysates of human liver tissue and human spleen tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ferritin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Ferritin in iBJ6 iPS Cell Line. Ferritin was detected in immersion fixed iBJ6 iPS cell line differentiated into hepatocytes using Mouse Anti-Human Ferritin Monoclonal Antibody (Catalog # MAB93541) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ferritin

FTH (Ferritin Heavy Chain 1; also Proliferation-Inducing Protein 15, PIG15, and PLIF) is a 183 aa cytosolic and secreted protein (SwissProt #: P02794). Human FTH is highly conserved among species and shares 93% and 95% aa identity with mouse and rat FTH, respectively. It is important for iron homeostatis by storing iron in a soluble form readily available to cells as well as having ferroxidase activity. Additionally, FTH plays a role in iron delivery to cells and can be secreted in small amounts in the serum as an iron carrier.

Entrez Gene IDs

2495 (Human)

Alternate Names

apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; Ferritin; ferritin, heavy polypeptide 1; FHC; FTHEC 1.16.3.1; FTHL6MGC104426; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15