Human FGF-17 Antibody

Catalog #: MAB319
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB319
MAB319-SP

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All FGF-17 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISA, approximately 7% cross-reactivity with recombinant human (rh) FGF-5 is observed and no cross-reactivity with rhFGF-acidic, rhFGF-basic, rhFGF-4, rhFGF-6, rhFGF-7, rhFGF8b, rhFGF-9, rhFGF-10, rhFGF-17, rhFGF-18, rhFGF-19, recombinant mouse (rm) FGF-8b, rmFGF-8c and rmFGF-15 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 121630

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human FGF-17
Thr23-Thr216
Accession # O60258

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human FGF‑17 (Catalog # 319-FG)

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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FGF‑17 in Human Kidney. FGF‑17 was detected in immersion fixed paraffin-embedded sections of human kidney using Human FGF‑17 Monoclonal Antibody (Catalog # MAB319) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the basement membrane supporting epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-17

Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.

References

Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
Xu, J. et al. (1999) Mech. Dev. 83:165.

Long Name

Fibroblast Growth Factor 17

Entrez Gene IDs

8822 (Human); 14171 (Mouse)

Alternate Names

FGF-13; FGF17; FGF-17; fibroblast growth factor 17

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human FGF-17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Klotho Coreceptors Inhibit Signaling by Paracrine Fibroblast Growth Factor 8 Subfamily Ligands
Authors: Regina Goetz, Mutsuko Ohnishi, Xunshan Ding, Hiroshi Kurosu, Lei Wang, Junko Akiyoshi et al.
Molecular and Cellular Biology
Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes.
Authors: Artavanis-Tsakonas K, Eleme K, McQueen KL, Cheng NW, Parham P, Davis DM, Riley EM
J. Immunol., 2003-11-15;171(10):5396-405.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry