Human FGF-18 Antibody

Catalog #: MAB6672 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6672-100
MAB6672-SP

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All FGF-18 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-18 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-18 by direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 91815

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived human FGF-18 protein
Glu28-Ala207
Accession # O76093

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human FGF‑18 Monoclonal Antibody (Catalog # MAB667).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human FGF‑18 ELISA Standard Curve. Recombinant Human FGF‑18 protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF‑18 Monoclonal Antibody (MAB667) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human FGF‑18 Monoclonal Antibody (Catalog # MAB6672) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-18

Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).

References