Human FGF-20 Antibody Summary
Met1-Thr211
Accession # Q9NP95
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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FGF‑20 in SW480 Human Cell Line. FGF-20 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Rat Anti-Human FGF-20 Monoclonal Antibody (Catalog # MAB2547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-20
Fibroblast growth factor 20 (FGF-20) is a member of the FGF gene family, which currently contains 22 members. Based on its structure, it is further classified as an FGF-9 subfamily member. All FGF family members are heparin-binding growth factors with a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1). The cDNA of FGF-20 predicts a 211 aa polypeptide without a canonical signal peptide sequence, a feature shared with other members of this subfamily (2‑4). Nevertheless, it is secreted with a molecular weight of 27 kDa (2‑4). FGF-20 is known to bind to heparin (4). No alternate splice forms have been reported. However, three amino acid polymorphisms are known, and single nucleotide polymorphisms in noncoding regions that may effect expression show a strong correlation with a risk of developing Parkinson’s disease (5, 6). Human FGF-20 shows 98% aa identity to bovine FGF-20 and 95% aa identity to both rat and mouse FGF-20. Within the FGF-9 subfamily, FGF-20 is 69% and 63% aa identical to human FGF-9 and FGF-16, respectively. Human FGF-20 is reported to be promiscuous in its selection of receptors which include FGF R1c, FGF R2c, FGF R3b, FGF R3c and FGF R4 (4, 7, 8). FGF-20 is expressed a variety of cells, including dopaminergic neurons (2), fibroblasts, keratinocytes and breast epithelium (4), and multiple sites in the fetus (2, 7). Finally, the expression of FGF-20 and DKK-1 is regulated by beta -catenin during development and tumorigenesis, implying that FGF-20 may play a role in the oncogenesis induced by the Wnt signaling pathway (9).
- Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
- Ohmachi, S. et al. (2000) Biochem. Biophys. Res. Commun. 277:355.
- Kirikoshi, H. et al. (2000) Biochem. Biophys. Res. Commun. 274:337.
- Jeffers, M. et al. (2001) Cancer Res. 61:3131.
- van der Walt, J. et al. (2004) Am. J. Hum. Genet. 74:1121.
- Genbank Accession # Q9NP95.
- Lavine, K.J. et al. (2005) Dev. Cell 8:85.
- Ohmachi, S. et al. (2003) J. Neurosci. Res. 72:436.
- Chamorro, M.N. et al. (2005) EMBO J. 24:73.
Product Datasheets
Citation for Human FGF-20 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Variation in the miRNA-433 binding site of FGF20 confers risk for Parkinson disease by overexpression of alpha-synuclein.
Authors: Wang G, van der Walt JM, Mayhew G, Li YJ, Zuchner S, Scott WK, Martin ER, Vance JM
Am. J. Hum. Genet., 2008-01-31;82(2):283-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Sandwich ELISA. Tested against hFGF20 standards (800 pg/ml-25 pg/ml), run in duplicate.
