Human FGF-22 Antibody

Catalog #: MAB3867 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3867
MAB3867-SP

Cell Proliferation Induced by FGF‑22 and Neutralization by Human FGF‑22 Antibody.

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All FGF-22 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-22 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-22 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FGF-basic, rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, -20, -21, -23, recombinant mouse (rm) FGF-basic, rmFGF-8C, or -15 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 435008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FGF-22
Thr23-Ser170
Accession # Q9HCT0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human FGF‑22 (Catalog # 3867-FG)

Neutralization

Measured by its ability to neutralize FGF‑22-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND₅₀) is typically 1.5-7.5 µg/mL in the presence of 1 µg/mL Recombinant Human FGF‑22.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by FGF‑22 and Neutralization by Human FGF‑22 Antibody. Recombinant Human FGF-22 (Catalog # 3867-FG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-22 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF-22 Monoclonal Antibody (Catalog # MAB3867). The ND₅₀ is typically 1.5-7.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-22

Fibroblast growth factor-22 (FGF-22) is a 23 kDa, non-glycosylated member of the FGF-7 subfamily, from the FGF family of heparin-binding growth factors (1‑3). The human FGF-22 precursor is 170 amino acids (aa) in length, and contains a 22 aa signal sequence with a 148 aa mature region (4‑6). The mature region shows a centrally-placed, 120 aa beta -trefoil region (aa 43‑168) that is characteristic of all FGF family members. Human FGF-22 potentially has one alternate splice form. This isoform is 129 aa in length, and shows a 31 aa substitution for the first N-terminal 72 aa of the standard, or long, form (7). There is no information related to its possible function. Mature human FGF-22 is 86% aa identical to mouse FGF-22, with the mouse molecule showing a 9 aa deletion at the N-terminus (5). FGF-22 is synthesized by at least three cell types; keratinocytes, neurons, and skeletal muscle myotubes (4, 8, 9). In neurons and myotubes, FGF-22 is presumed to function as an organizer of the presynaptic apparatus. Expressed by postsynaptic (or target) cells, FGF-22 is believed to bind to FGF R2b on the surface of innervating processes, resulting in synaptic vesicle clustering, organization, and neurite branching (8, 10). Although FGF-22 is assumed to be secreted, little can be found in expressing cell culture media. Presumably, it is bound to 34 kDa FGF-BP1, which is a molecule described as typically associated with cell membrane proteoglycans (6, 11). Thus, following secretion, FGF-22 could quickly be immobilized by FGF-BP1, only to be released at a later time, or aided by FGF-BP1 in its interaction with FGF R2b (6, 10, 11).

References