Human FGL2 Antibody

Catalog #: MAB5974 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5974
MAB5974-SP

Detection of Human FGL2 by Western Blot.

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Human FGL2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGL2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-like 2, and recombinant mouse Angiopoietin-3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 532223

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FGL2
Asn24-Pro439
Accession # Q14314

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FGL2 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FGL2 Monoclonal Antibody (Catalog # MAB5974) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FGL2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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FGL2 in WI‑38 Human Cell Line. FGL2 was detected in immersion fixed WI-38 human fetal lung fibroblast cell line using Mouse Anti-Human FGL2 Monoclonal Antibody (Catalog # MAB5974) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGL2

FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64‑70 kDa secreted glycoprotein of the fibrinogen-like superfamily. It is produced by macrophages and endothelial cells in response to inflammatory mediators, T cells and fetal trophoblast cells. FGL2 has prothrombinase activity, promotes immune-mediated thrombosis and functions in transplant rejection and infection-related abortion. Mature human FGL2 gene is a 416 amino acid (aa) protein with a coiled-coil region and a fibronectin C-terminal homology domain or FRED. Coiled-coil dimers are covalently linked to form a tetrameric 260‑280 aa FGL2 complex. Mature human FGL2 shares 79% aa identity with mouse FGL2.

Long Name

Fibrinogen-like 2

Entrez Gene IDs

10875 (Human); 14190 (Mouse); 84586 (Rat); 102123631 (Cynomolgus Monkey)

Alternate Names

FGL2; fibrinogen-like 2; Fibrinogen-like protein 2; Fibroleukin; pT49T49; T49