Human FHL1 Antibody Summary
Thr93-Asn166
Accession # Q13642
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FHL1 by Western Blot. Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FHL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FHL1 in SH‑SY5Y Human Cell Line. FHL1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FHL1
FHL1 (Four-and-a-half LIM domains protein 1; also SLIM1 and KyoT1) is an approximately 35 kDa member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323 amino acids (aa) in length. It contains one C4-type Zn-finger region (aa 7-31), three distinct LIM domains (aa 40-77; 101-158; 162-212) and a bipartite NLS (aa 231-246). There is one 32 kDa splice variant that shows a 50 aa substitution for aa 231-323. This creates a fourth LIM domain. There are also alternative start sites 29 aa and 16 aa upstream of the standard site, a deletion of aa 168-296, and an 11 aa substitution for aa 81-91. Over aa 93-166, human FHL1 is 97% aa identical to mouse FHL1.
Product Datasheets
Citations for Human FHL1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Single-cell analysis of arthritogenic alphavirus-infected human synovial fibroblasts links low abundance of viral RNA to induction of innate immunity and arthralgia-associated gene expression
Authors: Fabian Pott, Dylan Postmus, Richard J. P. Brown, Emanuel Wyler, Elena Neumann, Markus Landthaler et al.
Emerging Microbes & Infections
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FHL1 is a major host factor for chikungunya virus infection
Authors: L Meertens, ML Hafirassou, T Couderc, L Bonnet-Mad, V Kril, BM Kümmerer, A Labeau, A Brugier, E Simon-Lori, J Burlaud-Ga, C Doyen, L Pezzi, T Goupil, S Rafasse, PO Vidalain, A Bertrand-L, L Gueneau, R Juntas-Mor, R Ben Yaou, G Bonne, X de Lamball, M Benkirane, P Roingeard, C Delaugerre, M Lecuit, A Amara
Nature, 2019-09-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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