Human FHL2 Antibody

Catalog #: AF4758 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4758
AF4758-SP

Detection of Human FHL2 by Western Blot.

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Human FHL2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FHL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human FHL1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FHL2
Lys167-Ile279
Accession # Q14192

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FHL2 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line and MG-63 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FHL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FHL2 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FHL2

FHL2 (Four-and-a-half LIM domains protein 2; also SLIM3 and DRAL) is a 30‑32 kDa member of the four-and-a half class of the LIM domain-only protein family. It shows a tissue-specific expression pattern (myocardium; skeletal muscle; prostatic epithelium), and is noted for its strong transactivation of the androgen receptor (AR). Human FHL2 is 279 amino acids (aa) in length. It contains four distinct LIM domains (aa 40‑92; 101‑153; 162‑212; 221‑275) that all bind to the AR. There are two potential alternate start sites (Met115 and Met110) and one splice variant that shows a 99 aa substitution for aa 53‑279. Over aa 167‑279, human FHL2 is 95% and 96% aa identical to canine and mouse FHL2, respectively.

Long Name

Four and a Half LIM Domains Protein 2

Entrez Gene IDs

2274 (Human); 14200 (Mouse); 63839 (Rat)

Alternate Names

AAG11; down-regulated in rhabdomyosarcoma LIM protein; DRAL; DRALAAG11; FHL2; FHL-2; four and a half LIM domains 2; four and a half LIM domains protein 2; four and a half LIM-domain protein 2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM3; SLIM-3; SLIM3aging-associated gene 11