Human Fibromodulin/FMOD Antibody

Catalog #: MAB5945 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5945
MAB5945-SP

Detection of Human Fibromodulin by Western Blot.

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Human Fibromodulin/FMOD Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fibromodulin/FMOD in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Lumican is observed.

Source

Monoclonal Mouse IgG_2B Clone # 549302

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fibromodulin/FMOD
Asp75-Ile376
Accession # NP_002014

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Fibromodulin by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Fibromodulin Monoclonal Antibody (Catalog # MAB5945) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fibromodulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibromodulin/FMOD

Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50‑70 kDa protein contains a sulfated N‑terminal complement C1q binding domain (aa 19‑98) and 12 leucine-rich repeats (aa 75‑376). A 40 kDa form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa identity with mouse or rat FMOD.

Entrez Gene IDs

2331 (Human); 14264 (Mouse); 64507 (Rat)

Alternate Names

Collagen-binding 59 kDa protein; fibromodulin proteoglycan; Fibromodulin; FM; FMOD; Keratan sulfate proteoglycan fibromodulin; SLRR2E; SLRR2EKSPG fibromodulin