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Human Fibronectin Antibody

Catalog #: MAB1918
16 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1918
MAB1918-SP
Detection of Human Fibronectin by Western Blot.
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Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fibronectin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fibronectin in Western blots.
Source
Monoclonal Mouse IgG1 Clone # P1H11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human plasma-derived Fibronectin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Yu, H. et al. (2004) Mol. Carcinog. 41:85.
 
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Fibronectin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western View Larger

Detection of Human Fibronectin by Simple WesternTM. Simple Western shows lysates of Exosome Standards (PC‑3) (NBP2-49856), Exosome Standards (Human Urine) (NBP2-49840) and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Fibronectin at approximately 290 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Immunocytochemistry Fibronectin antibody in HepG2 and Daudi Human Cell Lines by Immunocytochemistry (ICC). View Larger

Fibronectin in HepG2 and Daudi Human Cell Lines. Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Fibronectin antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Fibronectin in Human Breast Cancer Tissue. Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and fibroblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibronectin

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Entrez Gene IDs
2335 (Human); 280794 (Bovine)
Alternate Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

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Citations for Human Fibronectin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. MicroRNA let-7 downregulates STAT3 phosphorylation in pancreatic cancer cells by increasing SOCS3 expression
    Authors: Kripa Patel, Anita Kollory, Asami Takashima, Sibaji Sarkar, Douglas V. Faller, Sajal K. Ghosh
    Cancer Letters
  2. Heparanase promotes myeloma progression by inducing mesenchymal features and motility of myeloma cells
    Authors: Juan Li, Qianying Pan, Patrick D. Rowan, Timothy N. Trotter, Deniz Peker, Kellie M. Regal et al.
    Oncotarget
  3. Mesenchymal stem cells support human vascular endothelial cells to form vascular sprouts in human platelet lysate-based matrices
    Authors: S Summer, E Rossmanith, M Pasztorek, C Fiedler, M Gröger, S Rauscher, V Weber, MB Fischer
    PLoS ONE, 2022-12-15;17(12):e0278895.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Pharmacologic Targeting of MMP2/9 Decreases Peritoneal Metastasis Formation of Colorectal Cancer in a Human Ex Vivo Peritoneum Culture Model
    Authors: J Koch, D Mönch, A Maa beta, A Mangold, M Gužvi?, T Mürdter, T Leibold, MH Dahlke, P Renner
    Cancers, 2022-08-02;14(15):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells
    Authors: T Sakai, YY Choo, O Sato, R Ikebe, A Jeffers, S Idell, T Tucker, M Ikebe
    International Journal of Molecular Sciences, 2022-04-27;23(9):.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Whole Tissue
    Applications: IHC, Western Blot
  6. Diabetes Aggravates Post-ischaemic Renal Fibrosis through Persistent Activation of TGF-?1 and Shh Signalling
    Authors: DJ Kim, JM Kang, SH Park, HK Kwon, SJ Song, H Moon, SM Kim, JW Seo, YH Lee, YG Kim, JY Moon, SY Lee, Y Son, SH Lee
    Sci Rep, 2017-12-01;7(1):16782.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. PDL regeneration via cell homing in delayed replantation of avulsed teeth.
    Authors: Zhu W, Zhang Q, Zhang Y, Cen L, Wang J
    J Transl Med, 2015-11-14;13(0):357.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
    Authors: Hendel, Alon, Hsu, Ivy, Granville, David J
    Lab Invest, 2014-05-05;94(7):716-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
    Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
    Leukemia, 2011-12-02;26(5):1098-105.
    Species: Human
    Sample Types: Bone Marrow Fluid
    Applications: Neutralization
  10. Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
    Authors: Li H, Wang H, Wang F, Gu Q, Xu X
    PLoS ONE, 2011-08-10;6(8):e23322.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  11. Characterization and functional analysis of osteoblast-derived fibulins in the human hematopoietic stem cell niche.
    Authors: Hergeth SP, Aicher WK, Essl M, Schreiber TD, Sasaki T, Klein G
    Exp. Hematol., 2008-05-12;36(8):1022-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Interferon alpha-inducible protein 27 is an oncogene and highly expressed in cholangiocarcinoma patients with poor survival
    Authors: Chiang KC, Huang ST, Wu RC et al.
    Cancer Manag Res
  13. Elevated expression of microRNA‑378 in children with asthma aggravates airway remodeling by promoting the proliferation and apoptosis resistance of airway smooth muscle cells
    Authors: Peng Li, Xufang Lang, Shungang Xia
    Experimental and Therapeutic Medicine
  14. The KRAS-variant and its impact on normal breast epithelial cell biology
    Authors: Song-yi Jung, Poonam Malhotra, Kiana C. Nguyen, David Salzman, Yue Qi, Ethan H. Pak et al.
    Cell Death & Differentiation
  15. Glycosylated fibronectin as a first trimester marker for gestational diabetes
    Authors: Julia Alanen, Heidi Appelblom, Teemu Korpimaki, Heikki Kouru, Mikko Sairanen, Mika Gissler et al.
    Archives of Gynecology and Obstetrics
  16. Decorin-mediated suppression of tumorigenesis, invasion, and metastasis in inflammatory breast cancer
    Authors: Xiaoding Hu, Emilly S. Villodre, Richard Larson, Omar M. Rahal, Xiaoping Wang, Yun Gong et al.
    Communications Biology

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Reviews for Human Fibronectin Antibody

Average Rating: 4.6 (Based on 5 Reviews)

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Human Fibronectin Antibody
By Anonymous on 07/08/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cardiac fibroblasts Species: Human
Immunocytochemistry/Immunofluorescence Human Fibronectin Antibody MAB1918
Human Fibronectin Antibody
By Anonymous on 09/28/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: fibroblasts Species: Human
Immunocytochemistry/Immunofluorescence Human Fibronectin Antibody MAB1918
Human Fibronectin Antibody
By Anonymous on 05/15/2020
Application: ELISA Sample Tested: 293T human embryonic kidney cell line Species: Human

tested the antibody in whole cell ELISA using fibronectin overexpression cells. It works well.

ELISA Human Fibronectin Antibody MAB1918
Human Fibronectin Antibody
By Anonymous on 10/01/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human

Paired with AF1918

ELISA Human Fibronectin Antibody MAB1918
Human Fibronectin Antibody
By Anonymous on 08/12/2019
Application: WB Sample Tested: Purified protein Species: Human
Western Blot Human Fibronectin Antibody MAB1918