Home / FKBP52 / Human FKBP52 Antibody

Human FKBP52 Antibody

Catalog #: MAB4095
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4095
MAB4095-SP
Detection of Human FKBP52 by Western Blot.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FKBP52 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FKBP52 in Western blots. In Western blots, no cross-reactivity with other FKBP family members is observed.
Source
Monoclonal Rat IgG1 Clone # 425123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FKBP52
Met1-Ala459
Accession # Q02790
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human skin cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FKBP52 antibody by Western Blot. View Larger

Detection of Human FKBP52 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human FKBP52 Monoclonal Antibody (Catalog # MAB4095) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, 5 ng recombinant human FKBP38, FKBP51, and FKBP52 were included. A specific band for FKBP52 was detected at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP52

FK506 binding protein, also called FKBP52 and FKBP4, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans- proline residues critical for proper folding of proteins. It associates with HSP90 complexes that are critical for the proper folding of steroid receptors. FKBP52 knockout mice have abnormal reproductive organ development due to disruption of androgen receptor folding; and phosphorylation of FKBP52 at Thr143 seems important for steroid receptor activity.

Long Name
52 kDa FK506 Binding Protein
Entrez Gene IDs
2288 (Human); 14228 (Mouse); 260321 (Rat)
Alternate Names
51 kDa FK506-binding protein; 52 kDa FKBP; 59 kDa immunophilin; EC 5.2.1.8; FK506 binding protein 4, 59kDa; FK506-binding protein 4 (59kD); FK506-binding protein 4; FKBP4; FKBP-4; FKBP51; FKBP52; FKBP-52; FKBP52T-cell FK506-binding protein, 59kD; FKBP59; FKBP5952 kDa FK506-binding protein; HBI; HSP binding immunophilin; Hsp56; HSP-binding immunophilin; Immunophilin FKBP52; p52; p59; peptidyl-prolyl cis-trans isomerase FKBP4; peptidylprolyl cis-trans isomerase; PPIase FKBP4; PPIase; rotamase

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human FKBP52 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Hsp90 co-chaperones, FKBP52 and Aha1, promote tau pathogenesis in aged wild-type mice
    Authors: M Criado-Mar, NT Gebru, DM Blazier, LA Gould, JD Baker, D Beaulieu-A, LJ Blair
    Acta neuropathologica communications, 2021-04-08;9(1):65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Molecular Pathways Leading to Induction of Cell Death and Anti-Proliferative Properties by Tacrolimus and mTOR Inhibitors in Liver Cancer Cells
    Authors: E Navarro-Vi, P de la Cruz, L Contreras, R González, M Negrete, MA Rodríguez-, LM Marín-Góme, JM Álamo-Mart, A Calvo, MA Gómez-Brav, J de la Cruz, J Padillo, J Muntané
    Cell. Physiol. Biochem., 2020-05-06;54(3):457-473.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human FKBP52 Antibody

There are currently no reviews for this product. Be the first to review Human FKBP52 Antibody and earn rewards!

Have you used Human FKBP52 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review