Human FLI1 Antibody Summary
Ser13-Pro112
Accession # Q01543
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FLI1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.5 µg/mL of Human FLI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FLI1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FLI1
FLI1 (Friend leukemia virus integration site 1; also ERGB) is a member of the ETS family of transcription factors. Although its predicted MW is 51 kDa. FLI1 is expressed by megakaryocytes, macrophages, B cells and embryonic endothelial cells. It is a transcriptional activator that regulates the genes for Tie-2, GpIIb, MPL and TGF-beta RII. Human FLI1 is 452 amino acids (aa) in length. It contains one PNT domain that is involved in heterotypic interactions (aa 112-198) and an ETS DNA‑binding domain (aa 281-361). There are two potential splice variants. One shows an alternative start site at Met34, while another contains a 10 aa substitution for aa 1‑76. There are a series of naturally occurring 62-68 kDa chimeric proteins (EWS/FLI1) in tumors that result from a chromosomal translocation involving the EWS and FLI1 genes. One creates a 499 aa chimera that includes aa 1-265 of EWS and 220-452 of FLI1. Over aa 13-112, human FLI1 shares 98% aa identity with mouse FLI1.
Product Datasheets
Citations for Human FLI1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The role of FLI-1-EWS, a fusion gene reciprocal to EWS-FLI-1, in Ewing sarcoma
Authors: David J. Elzi, Meihua Song, Peter J. Houghton, Yidong Chen, Yuzuru Shiio
Genes & Cancer
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Constitutive activation of c-Abl/protein kinase C-delta/Fli1 pathway in dermal fibroblasts derived from patients with localized scleroderma.
Br. J. Dermatol., 2012-09-13;167(5):1098-105.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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