Human FLRT2 Antibody Summary
Cys36-Ser539
Accession # O43155
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FLRT2 by Western Blot. Western Blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human FLRT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2877) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FLRT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of Human FLRT2 by Simple WesternTM. Simple Western shows lysates of NCI‑H460 human large cell lung carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for FLRT2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FLRT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2877). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FLRT2
FLRT2 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85 kDa type I transmembrane (TM) human FLRT2 is synthesized as a 660 amino acid (aa) precursor with a 35 aa signal sequence, a 506 aa extracellular domain (ECD), a 21 aa TM segment and a 98 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT2 ECD shares 97%, 96%, 99%, 96% and 95% aa sequence identity with mouse, rat, equine, canine and bovine FLRT2 ECD, respectively. Human FLRT1 and FLRT3 ECDs share approximately 47% aa identity with FLRT2. The fibronectin domain of all three FLRTs can bind to FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). In adulthood, FLRT2 mRNA is most abundant in pancreas, but is also present in skeletal muscle, brain and heart (1). FLRT2 in mouse embryos shows highest expression in a subset of the sclerotome in the brain, the stomach, and posterior to the developing heart (2). This expression is distinct from that of FLRT1 and FLRT3 (2).
- Lacy, S. E. et al. (1999) Genomics 62:417.
- Haines, B. P. et al. (2006) Dev. Biol. 297:14.
- Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
- Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
- Ogata, S. et al. (2007) Genes Dev. 21:1817.
Product Datasheets
Citations for Human FLRT2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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LPHN2 inhibits vascular permeability by differential control of endothelial cell adhesion
Authors: Chiara Camillo, Nicola Facchinello, Giulia Villari, Giulia Mana, Noemi Gioelli, Chiara Sandri et al.
Journal of Cell Biology
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Synaptogenic activity of the axon guidance molecule Robo2 underlies hippocampal circuit function
Authors: H Blockus, SV Rolotti, M Szoboszlay, E Peze-Heids, T Ming, A Schroeder, N Apostolo, KM Vennekens, PS Katsamba, F Bahna, S Mannepalli, G Ahlsen, B Honig, L Shapiro, J de Wit, A Losonczy, F Polleux
Cell Reports, 2021-10-19;37(3):109828.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Placental labyrinth formation in mice requires endothelial FLRT2-UNC5B signaling
Authors: I Tai-Nagara, Y Yoshikawa, N Numata, T Ando, K Okabe, Y Sugiura, M Ieda, N Takakura, O Nakagawa, B Zhou, K Okabayashi, M Suematsu, Y Kitagawa, M Bastmeyer, K Sato, R Klein, S Navankasat, DY Li, S Yamagishi, Y Kubota
Development, 2017-06-02;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Tumor-specific inter-endothelial adhesion mediated by FLRT2 facilitates cancer aggressiveness
Authors: Ando T, Tai-Nagara I, Sugiura Y et al.
The Journal of clinical investigation
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