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Human Flt-3/Flk-2 Antibody

Catalog #: AF812
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF812
AF812-SP
Human Flt‑3/Flk‑2 ELISA Standard Curve.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Flt-3/Flk-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Flt‑3/Flk‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Flt-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Flt‑3/Flk‑2
Asn27-Asn541
Accession # AAA18947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Flt‑3/Flk‑2 Fc Chimera (Catalog # 368-ST)
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB8121).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Flt-3/Flk-2 DuoSet ELISA Kit (Catalog # DY812-05) for convenient development of a sandwich ELISA.

 
Flow Cytometry
2.5 µg/106 cells
THP‑1 human acute monocytic leukemia cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA View Larger

Human Flt‑3/Flk‑2 ELISA Standard Curve. Recombinant Human Flt‑3/Flk‑2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (MAB8121) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Flow Cytometry View Larger

Detection of Flt‑3/Flk‑2 in THP‑1 cells by Flow Cytometry THP‑1 cells were stained with Goat Anti-Human Flt‑3/Flk‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF812, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

References
  1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
  2. Drexler, H.G. (1996) Leukemia 10:588.
Long Name
fms-like Tyrosine Kinase 3
Entrez Gene IDs
2322 (Human); 14255 (Mouse)
Alternate Names
CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3

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Citations for Human Flt-3/Flk-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Soluble phosphorylated fms-like tyrosine kinase III. FLT3 protein in patients with acute myeloid leukemia (AML).
    Authors: Ravandi F, Jilani I, Estey E, Kantarjian H, Dey A, Aguilar C, Jitkaroon C, Giles F, O'Brien S, Keating M, Albitar M
    Leuk. Res., 2006-12-06;31(6):791-7.
    Species: Human
    Sample Types: Plasma
    Applications: Immunoprecipitation
  2. Inhibition of FLT3 in MLL. Validation of a therapeutic target identified by gene expression based classification.
    Authors: Armstrong SA, Kung AL, Mabon ME, Silverman LB, Stam RW, Den Boer ML, Pieters R, Kersey JH, Sallan SE, Fletcher JA, Golub TR, Griffin JD, Korsmeyer SJ
    Cancer Cell, 2003-02-01;3(2):173-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation

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