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Human Flt-3 Ligand/FLT3L Antibody

Catalog #: MAB308
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB308-SP
MAB308-100
MAB308-500
Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Flt-3 Ligand/FLT3L Antibody Summary

Species Reactivity
Human
Specificity
Detects human Flt-3 Ligand/FLT3L in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Flt‑3 Ligand/FLT3L is observed.
Source
Monoclonal Mouse IgG1 Clone # 40406
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt‑3 Ligand/FLT3L
Thr27-Pro185
Accession # AAA17999
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Flt‑3 Ligand/FLT3L (Catalog # 308-FK) under non-reducing conditions only.
Neutralization
Measured by its ability to neutralize Flt‑3 Ligand/FLT3L-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02‑0.06 µg/mL in the presence of 5 ng/mL Recombinant Human Flt‑3 Ligand/FLT3L.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody. View Larger

Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody. Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB308). The ND50 is typically 0.02-0.06 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.

Long Name
fms-like Tyrosine Kinase 3 Ligand
Entrez Gene IDs
2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)
Alternate Names
FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine

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Citations for Human Flt-3 Ligand/FLT3L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Single-Cell RNA-Seq Reveals AML Hierarchies Relevant to Disease Progression and Immunity
    Authors: Peter van Galen, Volker Hovestadt, Marc H. Wadsworth II, Travis K. Hughes, Gabriel K. Griffin, Sofia Battaglia et al.
    Cell
  2. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015-07-16;290(37):22338-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Long-term maintenance of gp120-specific immune responses by genetic vaccination with the HIV-1 envelope genes linked to the gene encoding Flt-3 ligand.
    Authors: Sailaja G, Husain S, Nayak BP, Jabbar AM
    J. Immunol., 2003-03-01;170(5):2496-507.
    Species: Human
    Sample Types:
    Applications: ELISA Development
  4. Peritonitis in the baboon: a primate model which stimulates human sepsis.
    Authors: Kinasewitz GT, 2016, Chang AC, Peer GT, Hinshaw LB, Taylor FB
    2000-02-01;13(2):100-9.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Plasma
    Applications: ELISA Development

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