Human Flt-3 Ligand/FLT3L Antibody

Catalog #: MAB308R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB308R-100
MAB308R-SP

Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Human Flt‑3 Ligand Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Flt-3 Ligand/FLT3L Antibody Summary

Species Reactivity

Human

Specificity

Detects human Flt-3 Ligand in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 40406R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand
Thr27-Pro185
Accession # AAA17999

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Flt‑3 Ligand-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02‑0.06 µg/mL in the presence of 5 ng/mL Recombinant Human Flt‑3 Ligand.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Human Flt‑3 Ligand Antibody. Recombinant Human Flt-3 Ligand (308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt-3 Ligand Monoclonal Antibody (Catalog # MAB308R). The ND₅₀ is typically 0.02-0.06 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Entrez Gene IDs

2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)

Alternate Names

FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine