Home / Follistatin-related Gene Protein/FLRG / Human Follistatin-related Gene Protein/FLRG Antibody

Human Follistatin-related Gene Protein/FLRG Antibody

Catalog #: AF1288
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1288
AF1288-SP
Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin-related Gene Protein/FLRG Antibody.
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Citations (2)
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Human Follistatin-related Gene Protein/FLRG Antibody Summary

Species Reactivity
Human
Specificity
Detects Follistatin-related Gene Protein/FLRG in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant mouse FLRG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FLRG
Met27-Val263
Accession # O95633
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

ELISA detection - This antibody can be used as a detection reagent in a human FLRG sandwich immunoassay in combination with the human FLRG capture reagent (Cat. # MAB1288) and recombinant human FLRG (Cat. # 1288-F3) as the standard. The suggested concentration range for this detection reagent is 0.1 - 0.4 μg/mL and should be titrated to determine the optimal concentration. In this format, less than 1% cross-reactivity is observed with rmFLRG.

 
Neutralization
Measured by its ability to neutralize Follistatin‑related Gene Protein/FLRG inhibition of Activin A-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.1 µg/mL Recombinant Human Follistatin‑related Gene Protein/FLRG and 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin-related Gene Protein/FLRG Antibody. View Larger

Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin-related Gene Protein/FLRG Antibody. Recombinant Human Follistatin-related Gene Protein/FLRG (Catalog # 1288-F3) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin-related Gene Protein/ FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1288). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-related Gene Protein/FLRG

Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta  and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).

References
  1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
  2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
  3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
  4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
  5. Bartholin, L. et al. (2001) Oncogene 20:5409.
Entrez Gene IDs
10272 (Human); 83554 (Mouse)
Alternate Names
FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3

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Citations for Human Follistatin-related Gene Protein/FLRG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks.
    Authors: Nanda S, Savvidou M, Syngelaki A, Akolekar R, Nicolaides KH
    Prenat. Diagn., 2010-12-28;31(2):135-41.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  2. FSTL3 is highly expressed in adipose tissue of individuals with overweight or obesity and is associated with inflammation
    Authors: Xiaoya Li, Hongwei Zhang, Xiaojing Ma, Yufei Wang, Xiaodong Han, Ying Yang et al.
    Obesity (Silver Spring)

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