Home / Follistatin-related Gene Protein/FLRG / Human Follistatin-related Gene Protein/FLRG Biotinylated Antibody

Human Follistatin-related Gene Protein/FLRG Biotinylated Antibody

Catalog #: BAF1288
3 Citations Datasheet / COA / SDS
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BAF1288
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Follistatin-related Gene Protein/FLRG Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Follistatin-related Gene Protein/FLRG in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant mouse FLRG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Follistatin-related Gene Protein/FLRG
Met27-Val263
Accession # O95633
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human Follistatin-related Gene Protein/FLRG Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Follistatin‑related Gene Protein/FLRG Antibody (Catalog # MAB1288)

Standard: Recombinant Human FLRG Protein (Catalog # 1288-F3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-related Gene Protein/FLRG

Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta  and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).

References
  1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
  2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
  3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
  4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
  5. Bartholin, L. et al. (2001) Oncogene 20:5409.
Entrez Gene IDs
10272 (Human); 83554 (Mouse)
Alternate Names
FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3

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Citations for Human Follistatin-related Gene Protein/FLRG Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Quantification of GDF11 and Myostatin in Human Aging and Cardiovascular Disease
    Authors: Marissa J Schafer
    Cell Metab, 2016-06-14;23(6):1207-15.
  2. Myostatin as a mediator of sarcopenia versus homeostatic regulator of muscle mass: insights using a new mass spectrometry-based assay
    Authors: H. Robert Bergen, Joshua N. Farr, Patrick M. Vanderboom, Elizabeth J. Atkinson, Thomas A. White, Ravinder J. Singh et al.
    Skeletal Muscle
  3. Maternal plasma levels of follistatin-related gene protein in the first trimester of pregnancies with Down syndrome.
    Authors: Miron P, Lambert J, Marcil A, Cowans NJ, Stamatopoulou A, Spencer K
    Prenat. Diagn., 2010-03-01;30(3):224-8.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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