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Human FoxO1/FKHR Antibody

Catalog #: MAB5939
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5939
MAB5939-SP
FoxO1/FKHR in LNCaP Human Cell Line.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxO1/FKHR Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxO1/FKHR in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxA1 (aa 253-472), B1 (aa 191-324), C1 (aa 208-322), D3 (aa 1-140), F1 (aa 129-354), G1 (aa 283-489), J1 (aa 301-421), J3 (aa 375-552), K1 (aa 493-670), M1 (aa 642-763), O3A (aa 372-673), P1 (aa 231-420), P2 (aa 269-460), P3 (aa 179-279), or P4 (aa 229-410) is observed.
Source
Monoclonal Mouse IgG2B Clone # 597554
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli- derived recombinant human FoxO1/FKHR
Ala353-Gly655
Accession # Q12778
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry FoxO1/FKHR antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC). View Larger

FoxO1/FKHR in LNCaP Human Cell Line. FoxO1/FKHR was detected in immersion fixed LNCaP human prostate cancer cell line using Human FoxO1/FKHR Monoclonal Antibody (Catalog # MAB5939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Detection of FoxO1/FKHR in Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells. FoxO1/FKHR was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells using Mouse Anti-Human FoxO1/FKHR Monoclonal Antibody (Catalog # MAB5939) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxO1/FKHR

FoxO1, also called FKHR (forkhead in ­rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity. A 60 kDa form, cleaved at R537, has been found in androgen-treated prostate cancer cells. Over aa 353-655, human FoxO1 shares 93% aa identity with mouse and rat FoxO1.

Long Name
Forkhead Box O1
Entrez Gene IDs
2308 (Human); 56458 (Mouse); 84482 (Rat)
Alternate Names
FKH1; FKHR; FKHRforkhead box protein O1; forkhead box O1; Forkhead box protein O1A; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; FoxO1; FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

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Citation for Human FoxO1/FKHR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neutrophils induce proangiogenic T cells with a regulatory phenotype in pregnancy
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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