Human FoxP1 Antibody

Catalog #: MAB4534 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4534
MAB4534-SP

Detection of Human FoxP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxP1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 542721

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FoxP1
Leu231-Gly420
Accession # Q9H334

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FoxP1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB4534) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific bands for FoxP1 were detected at approximately 60, 85, and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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FoxP1 in MCF‑7 Human Cell Line. FoxP1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB4534) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxP1

Forkhead Box P1 (FoxP1) is a member of the FOX family of transcription factors. Alternative splicing of human FoxP1 results in multiple transcript variants encoding different isoforms. FoxP1 contains both a DNA binding domain as well as protein‑protein interaction domains. FoxP1 can homo or heterodimerize with FoxP2 and FoxP4, with dimerization necessary for DNA binding and transcriptional activity. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 shows both oncogenic and tumor suppressive characteristics. Human isoforms of 489 to 677 amino acids contain alternate sequences within the first 60 amino acids and/or deletion of amino acids.

Long Name

Forkhead Box P1

Entrez Gene IDs

27086 (Human); 108655 (Mouse); 297480 (Rat)

Alternate Names

FLJ23741; fork head-related protein like B; forkhead box P1; forkhead box protein P1; FoxP1; glutamine-rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF1; QRF112CC4