Human FoxP1 Antibody

Detection of Human FoxP1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 25 µg of whole cell lysate (WCL) and 25 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for FoxP1 at approximately 65 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FoxP1 in BG01V Human Embryonic Stem Cells. FoxP1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

FoxP1 in Human Prostate. FoxP1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of FoxP1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with methanol.

Detection of Human FoxP1 by Simple Western^TM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for FoxP1 at approximately 99 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human FoxP1 by Immunocytochemistry/Immunofluorescence Spinal organoids consists of various spinal cord cell types.Representative images illustrating the presence of a HOXB4+ and b HOXC8+ cells in spinal organoids. Cellular nuclei were counterstained with DAPI. Scale bars, 100 μm. c Co-staining of FOXP1 (green) and ISL1 (red) demonstrates presence of limb-innervating neurons in spinal organoids. Scale bars, 100 μm. d Representative images of spinal organoids at day 42 stained with ISL1 (red) and ChAT (green). Cellular nuclei were counterstained with DAPI. Scale bars, 100 μm. Spinal organoids are stained with e CHX10+ cells (RED) and f CALB+ cells (green). Scale bars, 100 μm. g Co-staining of S100 beta and TUJ1 shows presence of astrocytes in spinal organoids. Scale bars, 100 μm. h Quantitative-PCR analysis demonstrates a lack of dorsal cell types in the spinal organoids generated Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30368521), licensed under a CC-BY license. Not internally tested by R&D Systems.