Human FoxP4 Antibody

Catalog #: AF6854 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6854
AF6854-SP

Detection of Human FoxP4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxP4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxP4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP2, rhFoxP3, and rhCTBP1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxP4
Pro549-Ser680
Accession # Q8IVH2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FoxP4 by Western Blot. Western blot shows lysates of COS‑7 African green monkey SV40 transformed kidney fibroblast-like cell line either mock transfected, transfected with human FoxP1, transfected with human FoxP2, transfected with human FoxP3, or transfected with human FoxP4. PVDF membrane was probed with 1:1000 dilution of Sheep Anti-Human FoxP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6854) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, the membrane was co-probed with an Anti-Actin Antibody. A specific band was detected for FoxP4 at approximately 75 kDa and Actin at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions.Image courtesy of Dr. Alison Banham, Ms. Linden Lyne, and Dr. Paola Bignone, The University of Oxford, Oxford, UK.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxP4

FoxP4 (Forkhead box protein P4; also FKHLA) is a 70-80 kDa member of the winged helix transcription factor gene family, FoxP subfamily of proteins. It is a transcriptional repressor that is strongly expressed in embryonic gut and lung epithelium, and in neurons both pre-and postnatally. Postnatally, it is found in cerebellar Purkinje cells. During development, it is found in hippocampal hilus, proliferating cells of the ventricular zone, and neurons of the basolateral nucleus of the amygdala. Human FoxP4 is 680 amino acids (aa) in length. It contains a large poly-Gln region (aa 65-219), a C2H2 Zn-finger domain (aa 307-332), a Leu-zipper dimerization motift (aa 349-370) and a forkhead DNA binding domain (aa 467-559). Phosphorylation occurs at Ser360. FoxP4 forms homodimers, and heterodimers with FoxP1 and FoxP2; it does not bind CTBP1. One potential splice form shows a deletion of aa 384-395. Over aa 549-680, human and mouse FoxP4 share 89% aa identity.

Long Name

Forkhead Box P4

Entrez Gene IDs

116113 (Human); 74123 (Mouse); 363185 (Rat)

Alternate Names

FKHLA; FLJ40908; FLJ44184; fork head-related protein like A; Fork head-related protein-like A; forkhead box P4; forkhead box protein P4; FoxP4; hFKHLA; winged-helix repressor FOXP4