Human FRA-1 Antibody

Detection of Human FRA‑1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 18 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human FRA-1 Polyclonal Antibody (Catalog # AF4935), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FRA-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FRA‑1 in Human Melanoma. FRA-1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human FRA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4935) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.