Human Frizzled-5 Antibody

Catalog #: AF1617
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1617
AF1617-SP

All Frizzled-5 Antibodies

Product Details

Citations (10)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Frizzled-5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Frizzled-5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Frizzled‑5
Ala27-Pro167
Accession # AAC50385

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Frizzled‑5 Fc Chimera (Catalog # 1617-FZ)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-5

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled (Frz) proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 amino acid (aa) and contains 10 invariant cysteines (1). Human Frz-5 shows 95% aa identity to mouse Frz-5 in the CRD region. In the mouse, Frz-5 is expressed in adult tissues (heart and kidney), as well as embryonic tissues (telencephalon, eye, and lung bud) (2, 3). Null mutations in Frz-5 reveal that it plays a role in the formation and maintenance of the extra-embryonic vasculature (3). Functional interactions with Frz-5 have been reported for Wnt-5a, Wnt-10b, Wnt-2b, and Wnt-7a, implicating these Wnts as ligands for the Frz-5 receptor (3-5).

Two distinct Wnt signal transduction pathways have been characterized. One is the canonical Wnt/ beta -catenin pathway that is involved in diverse biological mechanisms such as dorsal/ventral development in Xenopus embryos and mammalian tumor formation. Frz-5 is implicated in this pathway based on its ability to induce beta -catenin target genes in the presence of ligand (5). However, Frz-5 is also implicated in beta -catenin independent pathways (4, 6).

References

Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
Borello, U. et al. (1999) Mech. Dev. 89:173.
Ishikawa, T. et al. (2001) Development 128:25.
He, X. et al. (1997) Science 275:1652.
Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
Veeman, M. et al. (2003) Dev. Cell 5:367.

Entrez Gene IDs

7855 (Human); 14367 (Mouse)

Alternate Names

C2orf31; chromosome 2 open reading frame 31; DKFZP434E2135; frizzled (Drosophila) homolog 5; frizzled homolog 5 (Drosophila); Frizzled5; Frizzled-5; Frz-5; FZ-5; FZD5; fzE5; HFZ5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Frizzled-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Secreted phosphoprotein-1 accelerates the progression of human colorectal cancer through activating &beta-catenin signaling
Authors: J Yi, Y Liu, L Zhang, C Fang
Oncology Letters, 2021-03-13;21(5):372.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Overexpression of G3BP1 facilitates the progression of colon cancer by activating &beta?catenin signaling
Authors: Y Li, J Wang, S Zhong, J Li, W Du
Molecular Medicine Reports, 2020-09-18;22(5):4403-4411.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
J Biol Chem, 2016-07-11;0(0):.
Species: Rat
Sample Types: Cell Lysates, Recombinant Protein
Applications: Bioassay, Western Blot
Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
PLoS ONE, 2014-08-29;9(8):e104937.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity.
Authors: Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ
PLoS ONE, 2011-08-17;6(8):e22920.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins.
Authors: Kilander MB, Dijksterhuis JP, Ganji RS, Bryja V, Schulte G
Cell. Signal., 2010-11-08;23(3):550-4.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Evidence for altered Wnt signaling in psoriatic skin.
Authors: Gudjonsson JE, Johnston A, Stoll SW
J. Invest. Dermatol., 2010-04-08;130(7):1849-59.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling.
Authors: Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST
J. Biol. Chem., 2008-01-30;283(14):9359-68.
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA Development
A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways
Authors: Tianzhi Huang, Angel A. Alvarez, Rajendra P. Pangeni, Craig M M. Horbinski, Songjian Lu, Sung-Hak Kim et al.
Nature Communications
THE CANONICAL WNT PATHWAY IN GASTRIC CARCINOMA
Authors: Levindo Alves de de OLIVEIRA, Celina Tizuko Fujiyama OSHIMA, Pedro Augusto SOFFNER, Marcelo de Souza SILVA, Rodrigo Rego LINS, Andréa Cristina de Moraes MALINVERNI et al.
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)