Human Frizzled-8 Antibody Summary
Ala25-Pro172
Accession # Q9H461
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Frizzled‑8 in PC‑3 Human Cell Line. Frizzled‑8 was detected in immersion fixed PC‑3 human prostate cancer cell line (positive staining) and U937 human histiocytic lymphoma cell line (negative control) using Mouse Anti-Human Frizzled‑8 Monoclonal Antibody (Catalog # MAB6129) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-8
Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1-2). Frizzled proteins are thought to be G-protein-coupled (1). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Human Frizzled-8 cDNA encodes 694 amino acids (aa) including a 27 aa signal peptide, a 247 aa extracellular domain (ECD), the 7TM region (aa 276-605), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa 606-694) (2). The ECD includes a cysteine-rich region (CRD, aa 30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa 28-172, human Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with mouse, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 3). During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis (4). Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate beta -catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein (4) or CTGF (connective tissue growth factor) inhibits Wnt signaling (5-7). These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas (5-10).
- Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
- Saitoh, T. et al. (2001) Int. J. Oncol. 18:991.
- Dann, C.E. et al. (2001) Nature 412:86.
- Lu C.C. et al. (2004) Gene Expr. Patterns 4:569.
- Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
- Zhu, W. et al. (2008) Nature 454:345.
- Mercurio, S. et al. (2004) Development 131:2137.
- Chesnutt, C. et al. (2004) Dev. Biol. 274:334.
- Yamashita, J.K. et al. (2005) FASEB J. 19:1534.
- DeAlmeida, V.I. et al. (2007) Cancer Res. 67:5371.
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