Human FSH beta Antibody Summary
Asn19-Glu129
Accession # P01225
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FSH beta by Western Blot. Western blot shows lysates of human pituitary tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FSH beta Monoclonal Antibody (Catalog # MAB4310) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for FSH beta at approximately 17-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FSH beta
Follicle-stimulating hormone (FSH; also follitropin) is a 33 kDa heterodimer belonging to the glycoprotein hormones subunit beta family (1). The heterodimer is composed of two glycoproteins linked noncovalently: a common alpha subunit, which is also a component of luteinizing hormone, thyroid stimulating hormone, and chorionic gonadotropin; and a unique beta subunit that confers the protein’s specific biological action and is responsible for the interaction with FSH-receptor. The alpha subunit is 20 kDa and is synthesized as a 116 amino acid (aa) precursor that contains a 24 aa signal sequence and a 92 aa mature chain, which contains two potential sites of N-linked glycosylation (1-2). The beta subunit is 23 kDa and is synthesized as a 129 aa precursor with an 18 aa signal sequence and a 111 aa mature chain, which also contains two potential sites of N-linked glycosylation (1). Human FSH beta shares approximately 90% aa sequence identity with mouse and rat FSH beta. FSH is secreted by gonadotropes of the anterior pituitary gland. Its release is controlled by pulses of gonadotropin-releasing hormone (GnRH), and those pulses, in turn, are subject to the estrogen feedback from the gonads. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. It promotes follicle maturation and spermatogenesis through interactions with FSH-receptor.
- Van de Wiel, D.F. et al. (1998) J. Mol. Endocrinol. 20:83.
- Rathnam, P. and B.B. Saxena (1975) J. Biol. Chem. 250:6735.
Product Datasheets
Citation for Human FSH beta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Histone Methylations Define Neural Stem/Progenitor Cell Subtypes in the Mouse Subventricular Zone
Authors: Zhichao Zhang, Adeel Manaf, Yanjiao Li, Sonia Peña Perez, Rajikala Suganthan, John Arne Dahl et al.
Molecular Neurobiology
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
