Home / FSHR / Human FSHR Antibody

Human FSHR Antibody

Catalog #: MAB65591
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB65591
MAB65591-SP
Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FSHR Antibody Summary

Species Reactivity
Human
Specificity
Detects human FSH R in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 626717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FSH R
Accession # M65085
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize FSH alpha / beta -induced cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 1 ng/mL Recombinant Human FSH alpha / beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FSH R antibody in HEK293 Human Cell Line Transfected with Human FSH R and eGFP antibody by Flow Cytometry. View Larger

Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FSH R or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591) followed by APC-conjugated Goat anti-Mouse Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Neutralization cAMP Production Induced by FSH alpha / beta and Neutralization by Human FSH R Antibody. View Larger

cAMP Production Induced by FSH alpha / beta and Neutralization by Human FSH R Antibody. Recombinant Human FSH a/ beta (Catalog # 5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recom-binant Human FSH a/ beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND50 is typically 0.02-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FSHR

Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Long Name
Follicle Stimulating Hormone Receptor
Entrez Gene IDs
2492 (Human); 14309 (Mouse)
Alternate Names
follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH R; FSH receptor; FSHR; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human FSHR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Coprophagy Prevention Decreases the Reproductive Performance and Granulosa Cell Apoptosis via Regulation of CTSB Gene in Rabbits
    Authors: Guohua Song, Yadong Wang, Yaling Wang, Yixuan Jiang, Shuaijie Sun, Hanfang Cai et al.
    Frontiers in Physiology
  2. Modulation of Cathepsin S (CTSS) Regulates the Secretion of Progesterone and Estradiol, Proliferation, and Apoptosis of Ovarian Granulosa Cells in Rabbits
    Authors: Guohua Song, Yixuan Jiang, Yaling Wang, Mingkun Song, Xuanmin Niu, Huifen Xu et al.
    Animals (Basel)
  3. ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways
    Authors: F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol
    Oncogene, 2017-09-18;37(3):286-301.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. In vivo targeting of metastatic breast cancer via tumor vasculature-specific nano-graphene oxide
    Authors: Dongzhi Yang, Liangzhu Feng, Casey A. Dougherty, Kathryn E. Luker, Daiqin Chen, Meagan A. Cauble et al.
    Biomaterials

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human FSHR Antibody

There are currently no reviews for this product. Be the first to review Human FSHR Antibody and earn rewards!

Have you used Human FSHR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review